RT Journal Article
T1 Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis
A1 Zhu, Longfei
A1 Fernández Jiménez, Nadia
A1 Szymanska Lejmana, Maja
A1 Pelé, Alexandre
A1 Underwood, Charles J.
A1 Serra, Heïdi
A1 Lambing, Christophe
A1 Dluzewska, Julia
A1 Bieluszewski, Tomasz
A1 Pradillo Orellana, Mónica
A1 Henderson, Ian R.
A1 Ziolkowski, Piotr A.
AB The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1. The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants.
PB National Academy of Sciences
SN 0027-8424, ESSN: 1091-6490
YR 2021
FD 2021-08-12
LK https://hdl.handle.net/20.500.14352/4772
UL https://hdl.handle.net/20.500.14352/4772
LA eng
NO Unión Europea. Horizonte 2020
NO European Cooperation in Science and Technology
NO Ministerio de Educación
NO Polish National Science Center (NCN)
NO Foundation for Polish Science (FNP)
NO European Research Council/Biotechnology and Biological Sciences Research Council
DS Docta Complutense
RD 9 abr 2025