RT Journal Article
T1 The deletion of residues 268-292 of E1 impairs theability of HCV envelope proteins to induce pore formation
A1 Lombana, Laura
A1 Ortega Atienza, Sara
A1 Gómez Gutierrez, Julián
A1 Yélamos, Belén
A1 Peterson, Darrell
A1 Gavilanes, Francisco
AB We have obtained a chimeric protein containing the ectodomains of hepatitis C virus (HCV) envelope proteins but lacking the region 268-292 of E1. All its structural properties are coincident with those of the corresponding full length chimera. The deleted and entire chimeras were compared in terms of their membrane destabilizing properties. No differences were found in their ability to induce vesicle aggregation and lipid mixing but the deleted chimera showed a reduced capacity to promote leakage. The role of the deletion was also studied by obtaining HCV pseudoparticles (HCVpp). Both E1 and E2, and also the E1 deleted mutant, were incorporated into HCVpp to a similar level. However, HCVpp containing the E1 deleted protein are almost unable to infect Huh7 cells. These results point to the involvement of the region 268-292 in the formation of pores in the membrane necessary for the complete fusion of the membranes.
PB Elsevier
SN 1872-7492 (Online), 0168-1702 (print)
YR 2016
FD 2016-03-06
LK https://hdl.handle.net/20.500.14352/24382
UL https://hdl.handle.net/20.500.14352/24382
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 16 may 2024