RT Journal Article
T1 Dual Roles of Diadenosine Polyphosphates in Corneal Epithelial Cell Migration
A1 Mediero Muñoz, Aránzazu
A1 Peral Cerda, María Asunción
A1 Pintor Just, Jesús Jerónimo
AB Purpose. To investigate the influence of diadenosine polyphosphates on the rate of corneal epithelial cell migration.Methods. Primary corneal epithelial cell cultures were obtained from New Zealand White rabbits. Immunocytochemical experiments were performed by fixing the cells with 4% paraformaldehyde (PFA) and incubated with cytokeratin 3 primary antibody, which was subsequently incubated with a secondary IgG mouse labeled with FITC, and the cells were observed under confocal microscopy. Migration studies were performed by taking confluent monolayers that were wounded with a pipette tip and challenged with different di- and mononucleotides with or without P2 antagonist (n = 8 each treatment). For concentration–response analysis, compounds were tested in doses ranging from 10−8 to 10−3 M (n = 8). The stability of the dinucleotides was assayed by HPLC, with an isocratic method (n = 4).Results. Cells under study were verified as corneal epithelial cells via the immunocytochemical analysis. Cell migration experiments showed that Ap4A, UTP, and ATP accelerated the rate of healing (5, 2.75, and 3 hours, respectively; P < 0.05; P < 0.001), whereas Ap3A, Ap5A, and UDP delayed it (6.5, 10, and 2 hours, respectively; P < 0.05). ADP did not modify the rate of migration. Antagonists demonstrated that Ap4A and Ap3A did activate different P2Y receptors mediating corneal wound-healing acceleration and delay. Concerning the possible degradation of the dinucleotides, it was almost impossible to detect any products resulting from their cleavage.Conclusions. Based on the pharmacological profile of all the compounds tested, the two main P2Y receptors that exist in these corneal cells are a P2Y2 receptor accelerating the rate of healing and a P2Y6 receptor that delays this process.
PB The Association for Research in Vision and Ophthalmology, Inc.
SN 0146-0404
YR 2006
FD 2006-10
LK https://hdl.handle.net/20.500.14352/51960
UL https://hdl.handle.net/20.500.14352/51960
LA eng
NO Mediero Muñoz, A., Peral Cerda, M. A., Pintor Just, J. J. «Dual Roles of Diadenosine Polyphosphates in Corneal Epithelial Cell Migration». Investigative Opthalmology & Visual Science, vol. 47, n.o 10, octubre de 2006, p. 4500. DOI.org (Crossref), https://doi.org/10.1167/iovs.06-0209.
NO En O.A. en la web del editor.
DS Docta Complutense
RD 8 jun 2025