RT Journal Article
T1 Phytoglobins in the nuclei, cytoplasm and chloroplasts modulate nitric oxide signaling and interact with abscisic acid
A1 Rubio, Maria
A1 Calvo‐Begueria, Laura
A1 Díaz Mendoza, María Mercedes
A1 Elhiti, Mohamed
A1 Moore, Marten
A1 Matamoros, Manuel
A1 James, Euan
A1 Díaz, Isabel
A1 Pérez‐Rontomé, Carmen
A1 Villar, Irene
A1 Sein‐Echaluce, Violeta
A1 Hebelstrup, Kim
A1 Dietz, Karl‐Josef
A1 Becana, Manuel
AB Symbiotic hemoglobins provide O2 to N2-fixing bacteria within legume nodules, but the functions of non-symbiotic hemoglobins or phytoglobins (Glbs) are much less defined. Immunolabeling combined with confocal microscopy of the Glbs tagged at the C-terminus with green fluorescent protein was used to determine their subcellular localizations in Arabidopsis and Lotus japonicus. Recombinant proteins were used to examine nitric oxide (NO) scavenging in vitro and transgenic plants to show S-nitrosylation and other in vivo interactions with NO and abscisic acid (ABA) responses. We found that Glbs occur in the nuclei, chloroplasts and amyloplasts of both model plants, and also in the cytoplasm of Arabidopsis cells. The proteins show similar NO dioxygenase activities in vitro, are nitrosylated in Cys residues in vivo, and scavenge NO in the stomatal cells. The Cys/Ser mutation does not affect NO dioxygenase activity, and S-nitrosylation does not significantly consume NO. We demonstrate an interaction between Glbs and ABA on several grounds: Glb1 and Glb2 scavenge NO produced in stomatal guard cells following ABA supply; plants overexpressing Glb1 show higher constitutive expression of the ABA responsive genes Responsive to ABA (RAB18), Responsive to Dehydration (RD29A) and Highly ABA-Induced 2 (HAI2), and are more tolerant to dehydration; and ABA strongly upregulates class 1 Glbs. We conclude that Glbs modulate NO and interact with ABA in crucial physiological processes such as the plant's response to dessication
PB Wiley
SN 0960-7412
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/95097
UL https://hdl.handle.net/20.500.14352/95097
LA eng
NO Rubio, Maria C., et al. «Phytoglobins in the Nuclei, Cytoplasm and Chloroplasts Modulate Nitric Oxide Signaling and Interact with Abscisic Acid». The Plant Journal, vol. 100, n.o 1, octubre de 2019, pp. 38-54. https://doi.org/10.1111/tpj.14422.
NO L.C.-B. was the recipient of a predoctoral contract (FPI) from Ministerio de Economıa y Competitividad (MINECO). This work was supported byMINECO-Fondo Europeo de Desarrollo Regional (AGL2014-53717-RandAGL2017-85775-R).
NO Ministerio de Economía y Competitividad (España)
NO European Commission
DS Docta Complutense
RD 22 abr 2025