RT Journal Article
T1 Activity of lactic acid bacteria against biofilms formed by Staphylococcus aureus strains involved in acute mastitis
A1 Jurado Escobar, Rubén
A1 Jara Pérez, Josué
A1 Aragón, Alberto
A1 Hernando, Natalia
A1 Rodríguez Gómez, Juan Miguel
A1 Fernández Álvarez, Leonides
A1 Orgaz Martín, Belén
AB Staphylococcus aureus is the leading cause of acute mastitis during lactation. This species has a remarkable ability to form biofilms and to develop antibiotic resistance, which hampers the effectiveness of current therapeutic approaches. This study aims to evaluate the therapeutic potential of three lactic acid bacteria (LAB) strains (Limosilactobacillus fermentum I7, Limosilactobacillus reuteri 7SNG3-30 and Ligilactobacillus salivarius 22SNG3-30) to interfere with biofilms formed by two S. aureus strains isolated from milk of women with acute mastitis. Both live LAB cells and their respective cell-free supernatants were able to disrupt the S. aureus biofilm structure and significantly reduce its cellular viability. However, the effectiveness of these treatments was dependent on the S. aureus strain, the LAB strain, and the type of LAB preparation (active culture or cell-free supernatant) involved in each interaction. Overall, our results suggest that the tested LAB strains have the potential to be used either as probiotics or postbiotics complementing the current therapies against acute mastitis and other staphylococcal infections
PB Nature Research
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/128353
UL https://hdl.handle.net/20.500.14352/128353
LA eng
NO Jurado, R., Jara, J., Aragón, A., Hernando, N., Rodríguez, J. M., Fernández, L., & Orgaz, B. (2025). Activity of lactic acid bacteria against biofilms formed by Staphylococcus aureus strains involved in acute mastitis. Scientific reports, 15(1), 39980. https://doi.org/10.1038/s41598-025-23671-y
NO Author contributionsRJ conducted the main experiments, performed the data analysis and the figure layout and wrote the original  draft; JJ performed some of the experiments and contributed to the data analysis; AA and NH contributed to the data analysis; JMR wrote and reviewed the manuscript; LF and BO designed and supervised the work and wrote  and reviewed the manuscript
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Educación, Formación Profesional y Deportes (España)
DS Docta Complutense
RD 18 dic 2025