RT Journal Article
T1 Identification and characterisation of the proteins bound by specific phage‐displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts
A1 Cruz. Silvia de la, 
A1 Madrid García, Raquel
A1 García García, Aina
A1 Alcocer, Marcos
A1 Martín De Santos, María Del Rosario
A1 González Alonso, María Isabel
A1 García Lacarra, Teresa
AB BACKGROUND: Almonds and Brazil nuts are widely consumed allergenic nuts whose presence must be declared according to food labelling regulations. Their detection in food products has been recently achieved by ELISA methods with recombinant antibodies (scFv) isolated against complete Brazil nut and almond protein extracts. The screening of phage-scFv libraries against complete protein extracts confers a series of advantages over the use of purified proteins, as recombinant proteins might alter their native folding. However, using this strategy, the nature of the target detected by phage-displayed antibodies remains unknown, and requires further research to identify whether they are nut allergens or other molecules present in the extract, but not related to their allergenic potential.RESULTS: Electrophoretic, chromatographic, immunological and spectrometric techniques revealed that the Brazil nut (BE95) and almond (PD1F6 and PD2C9) specific phage-scFvs detected conformational epitopes of the Brazil nut and almond 11S globulins, recognised by WHO/IUIS as Ber e 2 and Pru du 6 major allergens. Circular dichroism data indicated that severe heat treatment would entail loss of epitope structure, disabling scFv for target detection.CONCLUSIONS: The presence of important Brazil nut and almond allergens (Ber e 2 and Pru du 6) in foodstuffs can be determined by using phage-display antibodies BE95, PD1F6 and PD2C9 as affinity probes in ELISA.
PB Wiley
SN 0022-5142
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/94201
UL https://hdl.handle.net/20.500.14352/94201
LA eng
NO de la Cruz, S., Madrid, R., García-García, A., Alcocer, M., Martín, R., González, I., & García, T. (2018). Identification and characterisation of the proteins bound by specific phage-displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts. Journal of the science of food and agriculture, 98(5), 1685–1695. https://doi.org/10.1002/jsfa.8640
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 28 abr 2025