RT Journal Article
T1 Candida genotyping of blood culture isolates from patients admitted to 16 hospitals in Madrid: genotype spreading during the COVID-19 pandemic driven by fluconazole-resistant C. parapsilosis
A1 Díaz García, Judith
A1 Gómez, Ana
A1 Machado, Marina
A1 Alcalá, Luis
A1 Reigadas Ramírez, Elena Manuela
A1 Sánchez Carrillo, Carlos
A1 Pérez Ayala, Ana
A1 Gómez García De La Pedrosa, María Elia
A1 González Romo, Fernando
A1 Cuétara, María Soledad
A1 García Esteban, Coral
A1 Quiles Melero, Inmaculada
A1 Zurita, Nelly Daniela
A1 Muñoz Algarra, María
A1 Durán Valle, María Teresa
A1 Sánchez García, Aída
A1 Muñoz García, Patricia Carmen
A1 Escribano, Pilar
A1 Guinea, Jesús
AB Background: Candidaemia and invasive candidiasis are typically hospital-acquired. Genotyping isolates from patients admitted to different hospitals may be helpful in tracking clones spreading across hospitals, especially those showing antifungal resistance. Methods: We characterized Candida clusters by studying Candida isolates (C. albicans, n = 1041; C. parapsilosis, n = 354, and C. tropicalis, n = 125) from blood cultures (53.8%) and intra-abdominal samples (46.2%) collected as part of the CANDIMAD (Candida in Madrid) study in Madrid (2019–2021). Species-specific microsatellite markers were used to define the genotypes of Candida spp. found in a single patient (singleton) or several patients (cluster) from a single hospital (intra-hospital cluster) or different hospitals (widespread cluster). Results: We found 83 clusters, of which 20 were intra-hospital, 49 were widespread, and 14 were intra-hospital and widespread. Some intra-hospital clusters were first detected before the onset of the COVID-19 pandemic, but the number of clusters increased during the pandemic, especially for C. parapsilosis. The proportion of widespread clusters was significantly higher for genotypes found in both compartments than those exclusively found in either the blood cultures or intra-abdominal samples. Most C. albicans- and C. tropicalis-resistant genotypes were singleton and presented exclusively in either blood cultures or intra-abdominal samples. Fluconazole-resistant C. parapsilosis isolates belonged to intra-hospital clusters harboring either the Y132F or G458S ERG11p substitutions; the dominant genotype was also widespread. Conclusions: the number of clusters—and patients involved—increased during the COVID-19 pandemic mainly due to the emergence of fluconazole-resistant C. parapsilosis genotypes.
PB MDPI
YR 2022
FD 2022-11-21
LK https://hdl.handle.net/20.500.14352/104771
UL https://hdl.handle.net/20.500.14352/104771
LA eng
NO Díaz-García J, Gómez A, Machado M, Alcalá L, Reigadas E, Sánchez-Carrillo C, et al. Candida Genotyping of Blood Culture Isolates from Patients Admitted to 16 Hospitals in Madrid: Genotype Spreading during the COVID-19 Pandemic Driven by Fluconazole-Resistant C. parapsilosis. JoF. 2022;8(11):1228.
NO 2022 Descuento MDPIFondos:Plan Nacional de I+D+I 2017-2020FEDER, ‘A way of making Europe'.Contrato Miguel ServetBeca predoctoral FIS
NO European Comission
NO Ministerio de Ciencia e Innovación
NO Instituto de Salud Carlos III
NO Instituto de Investigación Sanitaria Gregorio Marañón
DS Docta Complutense
RD 10 abr 2025