RT Journal Article
T1 Direct electrochemical biosensing in gastrointestinal fluids
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Sempionatto, Juliane
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
A1 Esteban Fernández de Ávila, Berta
A1 Wang, Joseph
AB Edible electrochemical biosensors with remarkable prolonged resistance to extreme acidic conditions are described for direct glucose sensing in gastrointestinal (GI) fluids of different pH ranges and compositions. Such direct and stable glucose monitoring is realized using carbon-paste biosensors prepared from edible materials, such as olive oil and activated charcoal, shown to protect the activity of the embedded glucose oxidase (GOx) enzyme from strongly acidic conditions. The enzymatic resistance to low-pH deactivation allowed performing direct glucose monitoring in strong acidic environments (pH 1.5) over a 90-min period, while the response of conventional screen-printed (SP) biosensors decreased significantly following 10-min incubation in the same fluid. The developed edible biosensor displayed a linear response between 2 and 10 mM glucose with sensitivity depending on the pH of the corresponding GI fluid. In addition, coating the electrode surface with pH-responsive enteric coatings (Eudragit® L100 and Eudragit® E PO), of different types and densities, allows tuning the sensor activation in gastric and intestinal fluids at specific predetermined times. The attractive characteristics and sensing performance of these edible electrochemical biosensors, along with their pH-responsive actuation, hold considerable promise for the development of ingestible devices towards the biosensing of diverse target analytes after prolonged incubation in challenging body fluids.
PB Springer Science and Business Media LLC
SN 1618-2642
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/94990
UL https://hdl.handle.net/20.500.14352/94990
LA eng
NO Ruiz-Valdepeñas Montiel V, Sempionatto JR, Campuzano S, Pingarrón JM, Esteban Fernández de Ávila B, Wang J. Direct electrochemical biosensing in gastrointestinal fluids. Anal Bioanal Chem. 2019 Jul;411(19):4597-4604. doi: 10.1007/s00216-018-1528-2. Epub 2018 Dec 14. PMID: 30552492.
NO University of California San Diego
DS Docta Complutense
RD 27 abr 2026