RT Journal Article
T1 Tuning Almond Lipase Features by Using Different Immobilization Supports
A1 Cherni, Oumaima
A1 Carballares Navarro, Diego
A1 Siar, El Hocine
A1 Abellanas Pérez, Pedro
A1 de Andrades, Diandra
A1 Rocha Martín, Javier
A1 Bahri, Sellema
A1 Fernandez Lafuente, Roberto
AB The lipase from Prunus dulcis almonds has been immobilized for the first time. For this purpose, two different supports, an octadecyl methacrylate particulate support, and aminated agarose (monoaminoethyl-N-aminoethyl) have been utilized. Both immobilized biocatalysts show improved enzyme stability, but great changes in enzyme specificity were detected. The enzyme immobilized via ion exchange maintained its activity intact versus p-nitrophenyl butyrate, while the enzyme immobilized on the hydrophobic support fully lost its activity versus this substrate, which was confirmed to be due to substrate adsorption on the support. However, this biocatalyst was much more active versus triacetin (more than 10-fold), R- or S- methyl mandelate at pH 7. At pH 9, a strong effect of using phosphate or bicarbonate as reaction buffers was detected. Using bicarbonate, the interfacially immobilized enzyme presented no activity versus R-isomer, but it was very active versus the S-isomer and triacetin. Using a phosphate buffer during the reaction, all compounds were recognized as substrates. The enzyme immobilized via ion exchange was significantly more active using phosphate; in fact, using bicarbonate, the enzyme was inactive versus both methyl mandelate isomers. This paper shows for the first time a great interaction between the effects of the immobilization protocol and buffer used during reaction on the enantiospecificity of lipases.
PB MDPI
SN 2073-4344
YR 2024
FD 2024-01-31
LK https://hdl.handle.net/20.500.14352/119549
UL https://hdl.handle.net/20.500.14352/119549
LA eng
NO Cherni, O., Carballares, D., Siar, E. H., Abellanas-Perez, P., de Andrades, D., Rocha-Martin, J., Bahri, S., & Fernandez-Lafuente, R. (2024). Tuning Almond Lipase Features by Using Different Immobilization Supports. Catalysts, 14(2). https://doi.org/10.3390/CATAL14020115
NO Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación (Spanish Government) (PID2022-136535OB-I00). The authors gratefully acknowledge FAPESP (São Paulo Research Foundation) by research scholarship to DA (Grant No: 2023/01338-7). Oumaima Cherni thanks the Tunisian Ministry of Higher Education and Scientific Research for her fellowship. The help and suggestions from Ángel Berenguer (Departamento de Química Inorgánica, Universidad de Alicante) are gratefully recognized.
NO Ministerio de Ciencia e Innovación (España)
NO Fundação de Amparo à Pesquisa do Estado de São Paulo (Brasil)
NO Ministère de l'Enseignement Supérieur et de la Recherche Scientifique da Tunísia
DS Docta Complutense
RD 19 dic 2025