RT Journal Article
T1 Increasing the magnesium concentration in various dialysate solutions differentially modulates oxidative stress in a human monocyte cell line
A1 Vida, Carmen
A1 Carracedo Añón, Julia María
A1 De Sequera Ortiz, Patricia
A1 Bodega, Guillermo
A1 Pérez, Rafael
A1 Alique, Matilde
A1 Ramírez, Rafael
AB Oxidative stress is exacerbated in hemodialysis patients by several factors, including the uremic environment and the use of dialysis fluids (DFs). Since magnesium (Mg) plays a key role in modulating immune function and in reducing oxidative stress, we aimed to evaluate whether increasing the Mg concentration in different DFs could protect against oxidative stress in immunocompetent cells in vitro. Effect of ADF (acetate 3 mM), CDF (citrate 1 mM), and ACDF (citrate 0.8 mM + acetate 0.3 mM) dialysates with Mg at standard (0.5 mM) or higher (1, 1.25, and 2 mM) concentrations were assessed in THP-1 monocyte cultures. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels were quantified under basal and uremic conditions (indoxyl sulfate (IS) treatment). Under uremic conditions, the three DFs with 0.5 mM Mg promoted higher ROS production and lipid damage than the control solution. However, CDF and ACDF induced lower levels of ROS and MDA, compared to that induced by ADF. High Mg concentration (1.25 and/or 2 mM) in CDF and ACDF protected against oxidative stress, indicated by reduced ROS and MDA levels compared to respective DFs with standard concentration of Mg. Increasing Mg concentrations in ADF promoted high ROS production and MDA content. Thus, an increase in Mg content in DFs has differential effects on the oxidative stress in IS-treated THP-1 cells depending on the dialysate used.
PB MDPI
SN ESSN 2076-3921
YR 2020
FD 2020-04-15
LK https://hdl.handle.net/20.500.14352/6486
UL https://hdl.handle.net/20.500.14352/6486
LA eng
NO Instituto de Salud Carlos III (ISCIII)/Fondo Europeo de Desarrollo Regional (FEDER)
NO Universidad Complutense de Madrid (UCM)/Banco de Santander
NO Universidad de Alcalá de Henares/Sociedad Española de Nefrología
DS Docta Complutense
RD 11 abr 2025