%0 Journal Article
%A Vetrone, F.
%A Naccache, R.
%A Zamarrón, A.
%A De La Fuente, A.J.
%A Sanz-Rodríguez, F.
%A Martínez Maestro, Laura
%A Rodriguez, E.M.
%A Jaque, D.
%A Sole, J.G.
%A Capobianco, J.A.
%T Temperature sensing using fluorescent nanothermometers
%D 2010
%@ 1936-0851
%U https://hdl.handle.net/20.500.14352/96493
%X Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.
%~