RT Journal Article
T1 Temperature sensing using fluorescent nanothermometers
A1 Vetrone, F.
A1 Naccache, R.
A1 Zamarrón, A.
A1 De La Fuente, A.J.
A1 Sanz-Rodríguez, F.
A1 Martínez Maestro, Laura
A1 Rodriguez, E.M.
A1 Jaque, D.
A1 Sole, J.G.
A1 Capobianco, J.A.
AB Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.
PB American Chemical Society
SN 1936-0851
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/96493
UL https://hdl.handle.net/20.500.14352/96493
LA eng
NO Fiorenzo Vetrone, Rafik Naccache, Alicia Zamarrón, Angeles Juarranz de la Fuente, Francisco Sanz-Rodríguez, Laura Martinez Maestro, Emma Martín Rodriguez, Daniel Jaque, José García Solé, and John A. Capobianco ACS Nano 2010 4 (6), 3254-3258 DOI: 10.1021/nn100244a
NO Universidad Autónoma de Madrid
NO Banco Santander
NO Natural Sciences and Engineering Research Council of Canada 
NO Ministere du Developpement economique, de l'Innovation et de l'Exportation (Quebec, Canada)
NO Comunidad de Madrid
NO Ministerio de Educación y Ciencia (España)
DS Docta Complutense
RD 26 feb 2026