RT Journal Article
T1 First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
A1 Gamella Carballo, María
A1 Laza, Anabel
A1 Parrón Ballesteros, Jorge
A1 Bueno Díaz, Cristina
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Pedrero Muñoz, María
A1 Bertolino, Franco
A1 Pingarrón Carrazón, José Manuel
A1 Villalba Díaz, María Teresa
A1 Campuzano Ruiz, Susana
AB A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds.
PB Elsevier
SN 1567-5394
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/73000
UL https://hdl.handle.net/20.500.14352/73000
LA eng
NO Gamella, Maria, et al. «First PCR-Free Electrochemical Bioplatform for the Detection of Mustard Sin a 1 Protein as a Potential “Hidden” Food Allergen». Bioelectrochemistry, vol. 150, abril de 2023, p. 108357. DOI.org (Crossref), https://doi.org/10.1016/j.bioelechem.2022.108357.
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 22 abr 2025