RT Journal Article
T1 The protein kinase Ire1 impacts pathogenicity of Candida albicans by regulating homeostatic adaptation to endoplasmic reticulum stress
A1 Sircaik, Shabnam
A1 Román González, Elvira
A1 Bapat, Priyanka
A1 Lee, Keunsook K
A1 Andes, David R
A1 Gow, Neil AR
A1 Nobile, Clarissa J
A1 Pla Alonso, Jesús
A1 Panwar, Sneh Lata
AB The unfolded protein response (UPR), crucial for the maintenance of endoplasmicreticulum (ER) homeostasis, is tied to the regulation of multiple cellular processes inpathogenic fungi. Here, we show that Candida albicans relies on an ER-resident protein, inositol-requiring enzyme 1 (Ire1) for sensing ER stress and activating the UPR.Compromised Ire1 function impacts cellular processes that are dependent on functional secretory homeostasis, as inferred from transcriptional profiling. Concordantly,an Ire1-mutant strain exhibits pleiotropic roles in ER stress response, antifungal toler-ance, cell wall regulation and virulence-related traits. Hac1 is the downstream targetof C. albicans Ire1 as it initiates the unconventional splicing of the 19 bp intron fromHAC1 mRNA during tunicamycin-induced ER stress. Ire1 also activates the UPR inresponse to perturbations in cell wall integrity and cell membrane homeostasis in a manner that does not necessitate the splicing of HAC1 mRNA. Furthermore, theIre1-mutant strain is severely defective in hyphal morphogenesis and biofilm formation as well as in establishing a successful infection in vivo. Together, these findings demonstrate that C. albicans Ire1 functions to regulate traits that are essential for virulence and suggest its importance in responding to multiple stresses, thus integrating various stress signals to maintain ER homeostasis.
PB Wiley
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/129356
UL https://hdl.handle.net/20.500.14352/129356
LA eng
NO Sircaik S, Román E, Bapat P, et al. The protein kinase Ire1 impacts pathogenicity of Candida albicans by regulating homeostatic adaptation to endoplasmic reticulum stress. Cellular Microbiology. 2021; 23:e13307. https://doi.org/10.1111/cmi.13307
NO Defence Research and Development Organization (India)
DS Docta Complutense
RD 27 feb 2026