RT Journal Article
T1 Neoadjuvant Intratumoral Immunotherapy with Cowpea Mosaic Virus Induces Local and Systemic Antitumor Efficacy in Canine Mammary Cancer Patients
A1 Valdivia Lara, Edgar Guillermo
A1 Alonso Miguel, Daniel
A1 Pérez Alenza, María De Los Dolores
A1 Zimmermann, Anna Barbara Emilia
A1 Schaafsma, Evelien
A1 Kolling IV, Fred W.
A1 Barreno San Antolín, Lucía
A1 Alonso Díez, Ángela
A1 Beiss, Veronique
A1 Affonso de Oliveira, Jessica Fernanda
A1 Suárez Redondo, María
A1 Fiering, Steven
A1 Steinmetz, Nicole F.
A1 Vom Berg, Johannes
A1 Peña Fernández, Laura Luisa
A1 Arias Pulido, Hugo
AB The lack of optimal models to evaluate novel agents is delaying the development of effective immunotherapies against human breast cancer (BC). In this prospective open label study, we applied neoadjuvant intratumoral immunotherapy with empty cowpea mosaic virus-like particles (eCPMV) to 11 companion dogs diagnosed with canine mammary cancer (CMC), a spontaneous tumor resembling human BC. We found that two neoadjuvant intratumoral eCPMV injections resulted in tumor reduction in injected tumors in all patients and in noninjected tumors located in the ipsilateral and contralateral mammary chains of injected dogs. Tumor reduction was independent of clinical stage, tumor size, histopathologic grade, and tumor molecular subtype. RNA-seq-based analysis of injected tumors indicated a decrease in DNA replication activity and an increase in activated dendritic cell infiltration in the tumor microenvironment. Immunohistochemistry analysis demonstrated significant intratumoral increases in neutrophils, T and B lymphocytes, and plasma cells. eCPMV intratumoral immunotherapy demonstrated antitumor efficacy without any adverse effects. This novel immunotherapy has the potential for improving outcomes for human BC patients.
PB MDPI
YR 2023
FD 2023-09-08
LK https://hdl.handle.net/20.500.14352/104430
UL https://hdl.handle.net/20.500.14352/104430
LA eng
NO Valdivia, G.; AlonsoMiguel, D.; Perez-Alenza, M.D.; Zimmermann, A.B.E.; Schaafsma, E.; Kolling, F.W., IV; Barreno, L.; Alonso-Diez, A.; Beiss, V.; Affonso de Oliveira, J.F.; et al. Neoadjuvant Intratumoral Immunotherapy with Cowpea Mosaic Virus Induces Local and Systemic Antitumor Efficacy in Canine Mammary Cancer Patients. Cells 2023, 12, 2241. https://doi.org/ 10.3390/cells12182241
NO 2023 Descuento MDPI
NO Ministerio de Ciencia, Innovación y Tecnología (España)
DS Docta Complutense
RD 8 abr 2025