RT Journal Article
T1 Triterpene-Rich Supercritical CO2 Extracts from Apple By-product Protect Human Keratinocytes Against ROS
A1 Jimenez de la Peña Armada, Rocío Teresa
A1 Bronze, Maria Rosario
A1 Matias, Ana
A1 Mateos-Aparicio Cediel, Inmaculada
AB Supercritical fluid extraction, employing CO2 as solvent, was explored to recover high-value lipophilic compounds from apple by-product, the main solid residue generated after apple fruit processing. The experimental conditions were fixed at pressures of 300, 425, and 550 MPa and temperatures of 37, 46, and 55 °C. The total yield from the extractions was calculated and the antioxidant capacity of the different extracts was measured by ORAC and HORAC analytical methods. Furthermore, HPLCDAD-MS/MS and GLC analyses were performed, respectively, for triterpenic compounds and fatty acid identification. The extracts were tested over human keratinocyte cell (HaCaT) to evaluate their in vitro impact. Supercritical fluid extracts obtained at 46 °C exhibited the greater operational yield and the highest concentration of triterpenic acids, namely, betulinic acid, oleanolic acid, ursolic acid, uvaol/erythrodiol, and lupeol. These results support that apple by-product is a natural source of bioactive compounds with potential cosmeceutical applications.
PB Springer
SN 1935-5130
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/118099
UL https://hdl.handle.net/20.500.14352/118099
LA eng
NO De La Peña Armada R, Bronze MR, Matias A, Mateos-Aparicio I. Triterpene-rich supercritical co2 extracts from apple by-product protect human keratinocytes against ros. Food Bioprocess Technol [Internet]. mayo de 2021 [citado 14 de febrero de 2025];14(5):909-19. Disponible en: https://link.springer.com/10.1007/s11947-021-02615-0
NO Fundação para a Ciência e Tecnologia (Portugal)
NO Rede Nacional de Espectrometria de Massa (Portugal)
DS Docta Complutense
RD 22 abr 2025