RT Journal Article
T1 Interactions between TGF-beta1 and TGF-beta3 and their role in medial edge epithelium cell death and palatal fusion in vitro
A1 Murillo Arroyo, Francisco Javier
A1 Maldonado Bautista, Estela
A1 Barrio MC, 
A1 Río Sevilla, Aurora Del
A1 López, Y
A1 Martínez Sanz, Elena
A1 González, I
A1 Martín Álvaro, María Concepción
A1 Casado Gómez, Inmaculada
A1 Martínez Álvarez, María Concepción
AB In recent decades, studies have shown that both TGF-b1 and TGF-b3 play an important role in the induction of medial edge epithelium (MEE) cell death and palatal fusion. Many of these experiments involved the addition or blockage of one of these growth factors in wild-type (WT) mouse palate cultures, where both TGF-b1 and TGF-b3 are present. Few studies have addressed the existence of interactions between TGF-b1 and TGF-b3, which could modify their individual roles in MEE cell death during palatal fusion. We carried out several experiments to test this possibility, and to investigate how this could influence TGF-b1 and TGF-b3 actions on MEE cell death and palatal shelf fusion. We double immunolabelled developing mouse palates with anti-TGF-b1 or anti-TGF-b3 antibodies and TUNEL, added rhTGF-b1 or rhTGF-b3 or blocked the TGF-b1 and TGF-b3 action at different concentrations to WT or Tgf-b3 null mutant palate cultures, performed in situ hybridizations with Tgf-b1 or Tgf-b3 riboprobes, and measured the presence of TUNEL-positive midline epithelial seam (MES) cells and MES disappearance (palatal shelf fusion) in the different in vitro conditions. By combining all these experiments, we demonstrate great interaction between TGF-b1 and TGF-b3 in the developing palate and confirm that TGF-b3 has a more active role in MES cell death than TGF-b1, although both are major inductors of MES disappearance. Finally, the co-localization of TGF-b1, but not TGF-b3, with TUNEL in the MES allows us to suggest a possible role for TGF-b1 in MES apoptotic clearance.
PB Elsevier
SN 0301-4681
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/101450
UL https://hdl.handle.net/20.500.14352/101450
LA eng
NO Murillo, J., Maldonado, E., Barrio, M. C., Del Río, A., López, Y., Martínez-Sanz, E., ... & Martínez-Álvarez, C. (2009). Interactions between TGF-β1 and TGF-β3 and their role in medial edge epithelium cell death and palatal fusion in vitro. Differentiation, 77(2), 209-220.
NO Ministerio de Sanidad de España
NO Comunidad Autónoma de Madrid
NO Universidad Complutense
DS Docta Complutense
RD 19 jul 2024