RT Journal Article
T1 Assessment of sperm DNA fragmentation in stallion (Equus caballus) and donkey (Equus asinus) using the sperm chromatin dispersion test
A1 Cortés Gutiérrez, Elvira
A1 Crespo Castejón, Francisco
A1 Serres Dalmau, María Consolacion
A1 Gutiérrez de las Rozas, Ana Laura
A1 Dávila Rodríguez, Martha
A1 López Fernández, Carmen
A1 Gosálvez Berenguer, Jaime
AB Sperm DNA fragmentation (sDF) is an important parameter to assessing sperm quality. Information about sperm quality is not available for donkeys, especially in some breeds at risk of extinction. The objectives of this research were to test the four commercial variants of sperm chromatin dispersion test (SCD; sperm Halomax test), originally developed to assess sDF in boars, bulls, rams and stallions, in order to scrutinize their applicability in the study of sDF in a donkey breed at risk of extinction (Zamorano-Leonesa), for which there is no specific test available to analyze sperm at present. Only the SCD test, originally developed for stallions, produced stable and consistent results, and was deemed suitable to assess DNA fragmentation in sperm samples from donkeys. Image analysis was used to compare differences between the SCD methodology applied to stallion and donkey semen samples processed under the same experimental conditions. The extent of SCD in the SCD test was approximately 20% lower in donkey sperm than in stallion sperm. Yet, the ratio of chromatin sperm dispersion achieved in fragmented and unfragmented nuclei did not differ significantly between species. These data suggest that a similar protein depletion treatment can cause differences in protein removal in equivalent cells from different species and that sperm chromatin may be organized differently in stallions and donkeys.
PB Wiley
SN 0936-6768
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/97391
UL https://hdl.handle.net/20.500.14352/97391
LA eng
NO Cortés-Gutiérrez EI, Crespo F, Serres-Dalmau C, Gutiérrez de las Rozas AL, Dávila-Rodríguez MI, López-Fernández C, Gósalvez J. Assessment of sperm DNA fragmentation in stallion (Equus caballus) and donkey (Equus asinus) using the sperm chromatin dispersion test. Reprod Domest Anim. 2009 Oct;44(5):823-8
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 17 abr 2025