RT Journal Article
T1 Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
A1 Bautista Santa Cruz, José Manuel
A1 Puyet Catalina, Antonio
A1 Díez Martín, Amalia
A1 Reche Gallardo, Pedro Antonio
A1 Azcárate, Isabel G.
A1 Marín-García, Patricia
A1 Abad, Paloma
A1 Pérez-Benavente, Susana
A1 Paz-Artal, Estela
A1 Fobil, Julius N.
A1 Rubio, José M.
AB Incomplete non-sterile immunity to malaria is attained in endemic regions after recurrent infections by a large percentage of the adult population, who carry the malaria parasite asymptomatically. Although blood-stage Plasmodium falciparum rapidly elicits IgG responses, the target antigens of partially protective and non-protective IgG antibodies as well as the basis for the acquisition of these antibodies remain largely unknown. We performed IgG-immunomics to screen for P. falciparum antigens and to identify epitopes associated with exposure and clinical disease. Sera from malaria cases identified five prevalent antigens recognized by all analyzed patients' IgGs. Epitope mapping of them, using adult and children sera samples from an endemic malaria region in Ghana segregated into patients with positive or negative subclinical detection of P. falciparum, revealed binding specificity for two 20-mer immunodominant antigenic regions within the START-related lipid transfer protein and the protein disulfide isomerase PDI8. These 20-mer epitopes challenged with sera samples from children under 5 years old displayed specific IgG binding in those with detectable parasitemia, even at subclinical level. These results suggest that humoral response against START and PDI8 antigens may be triggered at submicroscopic parasitemia levels in children and may eventually be used to differentially diagnose subclinical malaria in children.
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/94128
UL https://hdl.handle.net/20.500.14352/94128
LA eng
NO Spanish-MINECO
DS Docta Complutense
RD 4 abr 2025