RT Journal Article
T1 Cerium dioxide-based nanostructures as signal nanolabels for current detection in the immunosensing determination of salivary myeloperoxidase
A1 Ramos López, Claudia
A1 García Rodrigo, Lorena
A1 Sánchez Tirado, Esther
A1 Agüí Chicharro, María Lourdes
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB This work reports the first electrochemical immunoplatform involving the use of synthesized cerium dioxide nanoparticles (CeO2NPs) / multi-walled carbon nanotubes (MWCNTs) composites as signal nanolabels for the determination of salivary myeloperoxidase (MPO), one of the proteins suggested as diagnostic biomarkers of periodontitis. The immunosensing device profits the benefits of CeO2NPs in terms of fast electron transfer and the catalytic activity and prevents the CeO2NPs tendency to agglomerate by decoration over MWCNTs, also providing high sensitivity. After a thorough characterization of the CeO2NPs/MWCNTs using various techniques and optimization of the variables involved in the preparation of the immunoplatform, a sandwich-type immunoassay was implemented with specific MPO capture and biotinylated detection antibodies. The use of horseradish peroxidase-streptavidin (HRP-Strept) or CeO2NPs/MWCNTs-(HRP-Strept) as labels for the amperometric detection at screen printed carbon electrodes (SPCEs) at −0.20 V vs. Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system, was compared. The method developed involving CeO2NPs/MWCNTs-(HRP-Strept) provided a linear calibration plot for MPO over the 1.0 to 100.0 ng mL−1 (R2 = 0.994) range with a limit of detection value of 0.40 ng mL−1. The immunoplatform was applied to the analysis of saliva from volunteers showing that the method allowed discrimination between healthy individuals and patients diagnosed with periodontitis through the determination of MPO in 1000-times diluted saliva. The obtained results agreed with those provided by an ELISA kit, this latter requiring a much longer assay time (4 h vs 2 h).
PB Elsevier
SN 0026-265X
YR 2024
FD 2024-06
LK https://hdl.handle.net/20.500.14352/113035
UL https://hdl.handle.net/20.500.14352/113035
LA eng
NO Claudia Ramos-López, Lorena García-Rodrigo, Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón, Cerium dioxide-based nanostructures as signal nanolabels for current detection in the immunosensing determination of salivary myeloperoxidase, Microchemical Journal, Volume 201, 2024, 110505, ISSN 0026-265X, https://doi.org/10.1016/j.microc.2024.110505. (https://www.sciencedirect.com/science/article/pii/S0026265X24006179)
NO Ministerio de Ciencia e Innovación
NO Comunidad de Madrid
DS Docta Complutense
RD 11 abr 2025