RT Journal Article
T1 Presence of the Ca2+-activated chloride channel anoctamin 1 in the urethra and its role in excitatory neurotransmission
A1 Sancho González, María
A1 García Pascual, María De Los Ángeles
A1 Triguero Robles, Domingo
AB We investigated the cellular distribution of the calcium-activated chloride channel (CaCC), anoctamin 1, in the urethra of mice, rats, and sheep by both immunofluorescence and PCR. We studied its role in urethral contractility by examining the effects of chloride-free medium and of several CaCC inhibitors on noradrenergic and cholinergic excitatory responses, and on nitrergic relaxations in urethral preparations. In all species analyzed, CaCC played a key role in urethral contractions, influencing smooth muscle cells activated by increases in intracellular calcium, probably due to calcium influx but with a minor contribution by IP3-mediated calcium release. The participation of CaCC in relaxant responses was negligible. Strong anoctamin 1 immunoreactivity was detected in the smooth muscle cells and urothelia of sheep, rat, and mouse urethra, but not in the interstitial cells of Cajal (ICC) in any of these species. RT-PCR confirmed the expression of anoctamin 1 mRNA in the rat urethra. This anoctamin 1 in urethral smooth muscle probably mediates the activity of chloride in contractile responses in different species, However, the lack of anoctamin 1 in ICCs challenges its proposed role in regulating urethral contractility in a manner similar to that observed in the gut.
PB AMER PHYSIOLOGICAL SOC
SN 1931-857X
SN 1522-1466
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/109871
UL https://hdl.handle.net/20.500.14352/109871
LA eng
NO Sancho M, García-Pascual A, Triguero D. Presence of the Ca2+-activated chloride channel anoctamin 1 in the urethra and its role in excitatory neurotransmission. Am J Physiol Renal Physiol. 2012;302(3):F390-400. doi: 10.1152/ajprenal.00344.2011.
NO Comunidad de Madrid–Universidad Complutense de Madrid
NO UCM-Santander
NO Fundación Mutua Madrileña
DS Docta Complutense
RD 9 abr 2025