RT Journal Article
T1 Comparative pangenomic analysis of Campylobacter fetus isolated from Spanish bulls and other mammalian species
A1 Pena‑Fernández, Nerea
A1 Ocejo, Medelin
A1 Van der Graaf‑van Bloois, Linda
A1 Lavín, José Luis
A1 Kortabarria, Nekane
A1 Collantes Fernández, Esther
A1 Hurtado, Ana
A1 Aduriz, Gorka
AB Campylobacter fetus comprises two closely related mammal-associated subspecies: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). The latter causes bovine genital campylobacteriosis, a sexually-transmitted disease endemic in Spain that results in significant economic losses in the cattle industry. Here, 33 C. fetus Spanish isolates were whole-genome sequenced and compared with 62 publicly available C. fetus genomes from other countries. Genome-based taxonomic identification revealed high concordance with in silico PCR, confirming Spanish isolates as Cff (n = 4), Cfv (n = 9) and Cfv biovar intermedius (Cfvi, n = 20). MLST analysis assigned the Spanish isolates to 6 STs, including three novel: ST-76 and ST-77 for Cfv and ST-78 for Cff. Core genome SNP phylogenetic analysis of the 95 genomes identified multiple clusters, revealing associations at subspecies and biovar level between genomes with the same ST and separating the Cfvi genomes from Spain and other countries. A genome-wide association study identified pqqL as a Cfv-specific gene and a potential candidate for more accurate identification methods. Functionality analysis revealed variations in the accessory genome of C. fetus subspecies and biovars that deserve further studies. These results provide valuable information about the regional variants of C. fetus present in Spain and the genetic diversity and predicted functionality of the different subspecies.
PB Springer
SN 2045-2322
YR 2024
FD 2024-02-22
LK https://hdl.handle.net/20.500.14352/103637
UL https://hdl.handle.net/20.500.14352/103637
LA eng
NO Author contributions: G.A. and A.H. conceived the study and participated in its design. N.P.F. and N.K. performed laboratory analysis. N.P.F., M.O., J.L.L. and L.G. participated in the bioinformatics analysis. N.P.F. wrote the manuscript, withinterpretation of results and discussion inputs from GA, AH, E.C., M.O., N.K., J.L.L. and L.G. All authors readand approved the fnal manuscript.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025