%0 Journal Article
%A Rinaldi, Francesca
%A Fernández-Lucas, Jesús
%A Fuente, Diego de la
%A Zheng, Changping
%A Bavaro, Teodora
%A Peters, Benjamin
%A Massolini, Gabriella
%A Annunziata, Francesca
%A Conti, Paola
%A Mata, Isabel de la
%A Terreni, Marco
%A Calleri, Enrica
%T Immobilized enzyme reactors based on nucleoside phosphorylases and 2′-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues
%D 2020
%@ 0960-8524
%U https://hdl.handle.net/20.500.14352/6667
%X In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2′-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2′-deoxy, 2′,3′- dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2′-deoxyuridine and 5-iodo-2′-deoxyuridine in 65–59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield).
%~