RT Journal Article
T1 Immobilized enzyme reactors based on nucleoside phosphorylases and 2′-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues
A1 Rinaldi, Francesca
A1 Fernández-Lucas, Jesús
A1 Fuente, Diego de la
A1 Zheng, Changping
A1 Bavaro, Teodora
A1 Peters, Benjamin
A1 Massolini, Gabriella
A1 Annunziata, Francesca
A1 Conti, Paola
A1 Mata, Isabel de la
A1 Terreni, Marco
A1 Calleri, Enrica
AB In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2′-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2′-deoxy, 2′,3′- dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2′-deoxyuridine and 5-iodo-2′-deoxyuridine in 65–59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield).
PB Elsevier
SN 0960-8524
YR 2020
FD 2020-03-26
LK https://hdl.handle.net/20.500.14352/6667
UL https://hdl.handle.net/20.500.14352/6667
LA eng
NO Cariplo Foundation (Italy)
DS Docta Complutense
RD 18 abr 2025