RT Journal Article
T1 Endogenous Hydrogen Sulfide has a Powerful Role in Inhibitory Neurotransmission to the Pig Bladder Neck
A1 Leite Fernandes, Vitor Samuel
A1 Fernandes Ribeiro, Ana Sofía
A1 Martínez, María Pilar
A1 Orensanz, Luis M.
A1 Barahona Gomáriz, María Victoria
A1 Martínez-Sáenz, Ana
A1 Recio Visedo, María Paz
A1 Benedito Castellote, Sara
A1 Bustamante, Salvador
A1 Carballido, Joaquín
A1 García Sacristán, Albino
A1 Prieto Ocejo, Dolores
A1 Hernández Rodríguez, Medardo Vicente
AB Purpose: We investigated the possible involvement of H2S in nitric oxide independent inhibitory neurotransmission to the pig bladder neck.Materials and methods: We used immunohistochemistry to determine the expression of the H2S synthesis enzymes cystathionine γ-lyase and cystathionine β-synthase. We also used electrical field stimulation and myographs for isometric force recordings to study relaxation in response to endogenously released or exogenously applied H2S in urothelium denuded, phenylephrine precontracted bladder neck strips under noradrenergic, noncholinergic, nonnitrergic conditions.Results: Cystathionine γ-lyase and cystathionine β-synthase expression was observed in nerve fibers in the smooth muscle layer. Cystathionine γ-lyase and cystathionine β-synthase immunoreactive fibers were also identified around the small arteries supplying the bladder neck. Electrical field stimulation (2 to 16 Hz) evoked frequency dependent relaxation, which was decreased by DL-propargylglycine and abolished by tetrodotoxin (blockers of cystathionine γ-lyase and neuronal voltage gated Na(+) channels, respectively). The cystathionine β-synthase inhibitor O-(carboxymethyl)hydroxylamine did not change nerve mediated responses. The H2S donor GYY4137 (0.1 nM to 10 μM) induced potent, concentration dependent relaxation, which was not modified by neuronal voltage gated Na(+) channels, or cystathionine γ-lyase or cystathionine β-synthase blockade.Conclusions: Results suggest that endogenous H2S synthesized by cystathionine γ-lyase and released from intramural nerves acts as a powerful signaling molecule in nitric oxide independent inhibitory transmission to the pig bladder neck.
PB Lippincott Williams & Wilkins
SN 0022-5347
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/100326
UL https://hdl.handle.net/20.500.14352/100326
LA eng
NO Ministerio de Ciencia e Innovación (España)
NO Fundação para a Ciência e Tecnologia(Portugal)
DS Docta Complutense
RD 14 dic 2025