RT Journal Article
T1 The fate of chondrocyte in osteoarthritic cartilage of transgenic mice expressing bovine GH
A1 Fernández-Criado, Carmen
A1 Martos-Rodríguez, Antonia
A1 Santos Álvarez, Inmaculada
A1 García-Ruíz, Josefa Predestinación
A1 Delgado-Baeza, Emilio
AB Objective: The aim of the present study was to demonstrate whether bGH transgenic mice develop OA. We therefore studied in this animal model the structural features of cartilage and the subchondral bone changes of the knee joints that may be associated with osteoarthritic lesion.Method: Degenerative changes in the knee joints of bGH transgenic female mice (N = 11) and control mice (N = 11) were histologically analyzed at the age of 7 months. Histochemical and stereological studies were conducted. Immunohistochemistry on cell cyclin activity (assessed by anti-PCNA labeling) and cell viability (assessed by bcl-2 expression), as well as ribosomal activity (AgNOR), TNF-alpha expression and apoptosis (TUNEL technique) were performed. In ten 7-month-old female mice (Tg+ N = 5; control N = 5) the knee articular cartilages were studied with electron microscopy techniques.Results: Disruption of the articular surface (18.2%), cleft (63.7%), cloning (81.8%), hypocellularity of chondrocytes (18.2%), moderate (54.6%) to severe (45.4%) loss of safranin-O staining, and duplication and rupture of the tidemark (54.5%) were some of the main features observed in articular cartilage chondrocytes of bGH transgenic mice. Furthermore, cell cyclin activity and cell viability decreased, while TNF-alpha expression and TUNEL+ cells increased. These chondrocytes also showed an increase in the number of black dots per cell, as revealed by the AgNOR technique.Conclusion: Our results show that bGH transgenic mice develop a lesion of the articular cartilage consistent with that described in osteoarthritis.
PB Elsevier
SN 1063-4584
YR 2004
FD 2004
LK https://hdl.handle.net/20.500.14352/96742
UL https://hdl.handle.net/20.500.14352/96742
LA eng
NO Fernández-Criado, C., Martos-Rodríguez, A., Santos-Alvarez, I., García-Ruíz, J. P., & Delgado-Baeza, E. (2004). The fate of chondrocyte in osteoarthritic cartilage of transgenic mice expressing bovine GH. Osteoarthritis and cartilage, 12(7), 543–551. https://doi.org/10.1016/j.joca.2004.04.002
DS Docta Complutense
RD 21 dic 2025