RT Journal Article
T1 Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper
A1 Mateescu, Bogdan
A1 Kowal, Emma
A1 Van Balkom, Bas
A1 Bartel, Sabine
A1 Bhattacharyya, Suvendra
A1 Buzás, Edit
A1 Buck, Amy
A1 Candia, Paola de
A1 Chow, Franklin
A1 Das, Saumya
A1 Driedonks, Tom
A1 Fernández Messina, Lola María
A1 Haderk, Franziska
A1 Hill, Andrew
A1 Jones, Jennifer
A1 Van Keuren‐Jensen, Kendall
A1 Lai, Charles
A1 Lässer, Cecilia
A1 di Liegro, Italia
A1 Lunavat, Taral
A1 Lorenowicz, Magdalena
A1 Maas, Sybren
A1 Mäger, Imre
A1 Mittelbrunn, Maria
A1 Momma, Stefan
A1 Mukherjee, Kamalika
A1 Nawaz, Muhammad
A1 Pegtel, Michiel
A1 Pfaffl, Michael
A1 Schiffelers, Raymond
A1 Tahara, Hidetoshi
A1 Théry, Clotilde
A1 Tosar, Juan Pablo
A1 Wauben, Marca
A1 Witwer, Kenneth
A1 Nolte‐'t Hoen, Esther
AB The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.
PB Wiley
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/97155
UL https://hdl.handle.net/20.500.14352/97155
LA eng
NO Mateescu, Bogdan, et al. «Obstacles and Opportunities in the Functional Analysis of Extracellular Vesicle RNA – an ISEV Position Paper». Journal of Extracellular Vesicles, vol. 6, n.o 1, diciembre de 2017, p. 1286095. https://doi.org/10.1080/20013078.2017.1286095.
NO AcknowledgementsWe thank all participants of the 2015 ISEV workshop on EV-RNA for their valuable input in roundtable discussions, which formed the basis for this position paper. We are grateful to Dr P. Vader for preparing Figure 3.
DS Docta Complutense
RD 7 abr 2025