RT Journal Article
T1 High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats
A1 Camiletti Moirón, Daniel
A1 Aparicio, Victoria
A1 Nebot Valenzuela, Elena
A1 Medina, Gerardo
A1 Martínez, Rosario
A1 Kapravelou, Garyfallia
A1 Andrade, Ana
A1 Porres, Jesús
A1 López Jurado, María
A1 Aranda, Pilar
AB We analyzed the effects of high-intensity exercise (HIE) and anabolic androgenic steroids (AAS) on brain redox status. 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10) with or without HIE and with or without weekly Stanozolol administration. Thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. Total superoxide dismutase (tSOD), manganese superoxide dismutase (Mn-SOD), copper/zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) activities were measured. Finally, protein expression levels of glutathione peroxidase (GPx), NAD(P)H dehydrogenase, Quinone 1 (NQO1), NF-E2-Related Factor 2 (Nrf2), glial fibrillary acidic protein (GFAP), nuclear factor kappa β p65 (NF-κβ) and signal transducer and activator of transcription 3 were determined. Brain PCC concentrations were lower in the HIE groups compared to the untrained controls, whereas CAT activity was higher (both, p<0.01). Both HIE and AAS groups exhibited higher expression levels of GFAP and GPx, but lower NQO1 levels (all, p<0.05). There were increased expression levels of NF-κβ in the AAS groups (p<0.01). In addition, there was increased expression of Nrf2 in the HIE groups (p<0.001). HIE*AAS interactions were found on TBARs content and GFAP expression, with HIE downregulating and upregulating AAS-mediated increases in TBARs and GFAP, respectively (p<0.05). Overall, HIE appeared to reduce the AAS-mediated negative effect on brain redox status.
PB Thieme Gruppe
SN 0172-4622
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/96367
UL https://hdl.handle.net/20.500.14352/96367
LA eng
NO Daniel Camiletti-Moirón, Virginia Aparicio, Elena Nebot, Gerardo Medina, Rosario Martínez, Garyfallia Kapravelou, Ana Andrade, Jesús Porres, Maria Lopez-Jurado, and Pilar Aranda Ramírez. High-intensity exercise modifies the effects of Stanozolol on brain oxidative stress in rats. International Journal of Sports Medicine. 2015 Mar 14; 36(10):1-8.
NO Ministerio de Ciencia e Innovación (España)
NO Ministerio de Educación (España)
DS Docta Complutense
RD 6 abr 2025