RT Journal Article
T1 Macrophage inflammatory and metabolic responses to graphene-based nanomaterials differing in size and functionalization
A1 Cicuéndez Maroto, Mónica
A1 Fernándes, Márcia
A1 Ayán Varela, Miguel
A1 Oliveira, Helena
A1 Feito Castellano, María José
A1 Díez Orejas, Rosalía María
A1 Paredes, Juan I.
A1 Villar Rodil, Silvia
A1 Vila, Mercedes
A1 Portolés Pérez, María Teresa
A1 Duarte, Iola F.
AB The preparation of graphene-based nanomaterials (GBNs) with appropriate stability and biocompatibility is crucial for their use in biomedical applications. In this work, three GBNs differing in size and/or functionalization have been synthetized and characterized, and their in vitro biological effects were compared. Pegylated graphene oxide (GO-PEG, 200–500 nm) and flavin mononucleotide-stabilized pristine graphene with two different sizes (PG-FMN, 200–400 nm and 100–200 nm) were administered to macrophages, chosen as cellular model due to their key role in the processing of foreign materials and the regulation of inflammatory responses. The results showed that cellular uptake of GBNs was mainly influenced by their lateral size, while the inflammatory potential depended also on the type of functionalization. PG-FMN nanomaterials (both sizes) triggered significantly higher nitric oxide (NO) release, together with some intracellular metabolic changes, similar to those induced by the prototypical inflammatory stimulus LPS. NMR metabolomics revealed that macrophages incubated with smaller PG-FMN displayed increased levels of succinate, itaconate, phosphocholine and phosphocreatine, together with decreased creatine content. The latter two variations were also detected in cells incubated with larger PG-FMN nanosheets. On the other hand, GO-PEG induced a decrease in the inflammatory metabolite succinate and a few other changes distinct from those seen in LPS-stimulated macrophages. Assessment of TNF-α secretion and macrophage surface markers (CD80 and CD206) further corroborated the low inflammatory potential of GO-PEG. Overall, these findings revealed distinct phenotypic and metabolic responses of macrophages to different GBNs, which inform on their immunomodulatory activity and may contribute to guide their therapeutic applications.
PB Elsevier
SN 0927-7765
YR 2020
FD 2020-02
LK https://hdl.handle.net/20.500.14352/6241
UL https://hdl.handle.net/20.500.14352/6241
LA eng
NO Cicuéndez Maroto, M., Fernándes, M., Ayán Varela, M. et al. «Macrophage Inflammatory and Metabolic Responses to Graphene-Based Nanomaterials Differing in Size and Functionalization». Colloids and Surfaces B: Biointerfaces, vol. 186, febrero de 2020, p. 110709. DOI.org (Crossref), https://doi.org/10.1016/j.colsurfb.2019.110709.
NO Ministerio de Economía, Comercio y Empresa (España)/Fondo Europeo de Desarrollo Regional
NO Ministerio de Ciencia, Innovación y Universidades (España)/Fondo Europeo de Desarrollo Regional
NO Principado de Asturias. Plan de Ciencia, Tecnología e Innovación
NO Aveiro Institute of Materials (Portugal)
NO Centro de Estudos do Ambiente e do Mar (Portugal)
NO European Union Framework Programme for Research and Innovation HORIZON 2020
NO Fundación para la Ciencia y la Tecnología (Portugal)
DS Docta Complutense
RD 2 oct 2024