RT Journal Article
T1 Unraveling the Structural Landscape of Chitosan-Based Heparan Sulfate Mimics Binding to Growth Factors: Deciphering Structural Determinants for Optimal Activity
A1 Revuelta Crespo, Julia
A1 Aranaz Corral, Inmaculada
A1 Acosta Contreras, Florentina Niuris
A1 Civera Tejuca, María Concepción
A1 Bastida Codina, Agatha
A1 Peña Montero, Nerea
A1 Toledo Monterrey, Dianelis
A1 Doncel Pérez, Ernesto
A1 Garrido Fernández, Leoncio
A1 Heras Caballero, Ángeles María
A1 García Junceda, Eduardo
A1 Fernández Mayoralas, Alfonso
AB Chitosan sulfates have demonstrated the ability to mimic heparan sulfate (HS) function. In this context, it is crucial to understand how the specific structural properties of HS domains determine their functionalities and biological activities. In this study, several HS-mimicking chitosans have been prepared to mimic the structure of HS domains that have proved to be functionally significant in cell processes. The results presented herein are in concordance with the hypothesis that sulfated chitosan–growth factor (GF) interactions are controlled by a combination of two effects: the electrostatic interactions and the conformational adaptation of the polysaccharide. Thus, we found that highly charged O-sulfated S-CS and S-DCS polysaccharides with a low degree of contraction interacted more strongly with GFs than N-sulfated N-DCS, with a higher degree of contraction and a low charge. Finally, the evidence gathered suggests that N-DCS would be able to bind to an allosteric zone and is likely to enhance GF signaling activity. This is because the bound protein remains able to bind to its cognate receptor, promoting an effect on cell proliferation as has been shown for PC12 cells. However, S-CS and S-DCS would sequester the protein, decreasing the GF signaling activity by depleting the protein or locally blocking its active site.
SN 1944-8244
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/96546
UL https://hdl.handle.net/20.500.14352/96546
LA eng
NO Revuelta, Julia, et al. «Unraveling the Structural Landscape of Chitosan-Based Heparan Sulfate Mimics Binding to Growth Factors: Deciphering Structural Determinants for Optimal Activity». ACS Applied Materials & Interfaces, vol. 12, n.o 23, junio de 2020, pp. 25534-45. DOI.org (Crossref), https://doi.org/10.1021/acsami.0c03074.
NO Banco Santander
DS Docta Complutense
RD 6 sept 2025