RT Journal Article
T1 Chlorpyrifos-induced cell proliferation in human breast cancer cell lines differentially mediated by estrogen and aryl hydrocarbon receptors and KIAA1363 enzyme after 24 h and 14 days exposure
A1 Moyano-Cires Ivanoff, Paula Viviana
A1 García Lobo, Jimena
A1 García Sánchez, José Manuel
A1 Pelayo A, 
A1 Muñoz Calero, P
A1 Frejo Moya, María Teresa
A1 Anadón Baselga, María José
A1 Lobo Alonso, Margarita
A1 Pino Sans, Javier Del
AB Organophosphate biocide chlorpyrifos (CPF) is involved with breast cancer. However, the mechanisms remain unknown. CPF increases cell division in MCF-7 cells, by estrogen receptor alpha (ERa) activation, although it is a weak ERa agonist, suggesting other mechanisms should be involved. Aromatic hydrocarbon receptor (AhR) activation increases cell division in human breast cancer cells, and CPF strongly activates it. Finally, the KIAA1363 enzyme, which is regulated by CPF, is overexpressed in cancer cells. Accordingly, we hypothesized that CPF or its metabolite chlorpyrifos-oxon (CPFO) could induce cell viability promotion in MCF-7 and MDA-MB-231 cell lines, through mechanisms related to ERa, AhR, and KIAA1363, after 24 h and 14 days treatment. Results show that, after acute and long-term treatment, CPF and CPFO alter differently KIAA1363, AhR, ER and cytochrome P450 isoenzyme 1A1 (CYP1A1) expression. In addition, they induced cell proliferation through ERa activation after 24 h exposure in MCF-7 cells and through KIAA1363 overexpression and AhR activation in MCF-7 and MDA-MB-231 cells after acute and long-term treatment. The results obtained in this work provide new information relative to the mechanisms involved in the CPF toxic effects that could lead to breast cancer disease.
PB Elsevier
SN 0045-6535
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/115463
UL https://hdl.handle.net/20.500.14352/115463
LA eng
NO Moyano, P., García, J., García, J. M., Pelayo, A., Muñoz-Calero, P., Frejo, M. T., Anadon, M. J., Lobo, M., & Del Pino, J. (2020). Chlorpyrifos-induced cell proliferation in human breast cancer cell lines differentially mediated by estrogen and aryl hydrocarbon receptors and KIAA1363 enzyme after 24 h and 14 days exposure. Chemosphere, 251. https://doi.org/10.1016/J.CHEMOSPHERE.2020.126426
NO CRediT authorship contribution statement:Paula Moyano: Methodology, Investigation, Writing - original draft, Writing - review & editing. Jimena García: Investigation, Validation. Jose Manuel García:   Validation, Writing - review & editing. Adela Pelayo: Resources, Supervision. Pilar Munoz- ~ Calero: Funding acquisition, Visualization. María Teresa Frejo: Formal analysis, Writing - review & editing. Maria Jose Anadon: Data curation, Writing - review & editing. Margarita Lobo: Formal analysis, Data curation. Javier Del Pino: Conceptualization, Methodology, Project administration, Supervision, Writing - review & editing.
NO Fundación Alborada( Universidad Complutense de Madrid)
DS Docta Complutense
RD 7 abr 2025