RT Journal Article
T1 Physical exercise shapes the mouse brain epigenome
A1 Urdinguio, Rocío G.
A1 Tejedor, Juan Ramon
A1 Fernández-Sanjurjo, Manuel
A1 Pérez, Raúl F.
A1 Peñarroya, Alfonso
A1 Ferrero, Cecilia
A1 Codina-Martínez, Helena
A1 Díez Planelles, Carlos
A1 Pinto Hernández, Paola
A1 Castilla Silgado, Juan
A1 Coto Vilcapoma, Almudena
A1 Díez Robles, Sergio
A1 Blanco Agudín, Noelia
A1 Tomás Zapico, Cristina
A1 Iglesias Gutiérrez, Eduardo
A1 Fernández García, Benjamín
A1 Fernandez, Agustin F.
A1 Fraga, Mario F.
AB Objective: To analyze the genome-wide epigenomic and transcriptomic changes induced by long term resistance or endurance training in the hippocampus of wild-type mice.  Methods: We performed whole-genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) of mice hippocampus after 4 weeks of specific training. In addition, we used a novel object recognition test before and after the intervention to determine whether the exercise led to an improvement in cognitive function.Results: Although the majority of DNA methylation changes identified in this study were training-model specific, most were associated with hypomethylation and were enriched in similar histone marks, chromatin states, and transcription factor biding sites. It is worth highlighting the significant association found between the loss of DNA methylation in Tet1 binding sites and gene expression changes, indicating the importance of these epigenomic changes in transcriptional regulation. However, endurance and resistance training activate different gene pathways, those being associated with neuroplasticity in the case of endurance exercise, and interferon response pathways in the case of resistance exercise, which also appears to be associated with improved learning and memory functions.Conclusions: Our results help both understand the molecular mechanisms by which different exercise models exert beneficial effects for brain health and provide new potential therapeutic targets for future research.
PB Elsevier
YR 2021
FD 2021-12-06
LK https://hdl.handle.net/20.500.14352/109878
UL https://hdl.handle.net/20.500.14352/109878
LA eng
NO Rocío G. Urdinguio, Juan Ramon Tejedor, Manuel Fernández-Sanjurjo, Raúl F. Pérez, Alfonso Peñarroya, Cecilia Ferrero, Helena Codina-Martínez, Carlos Díez-Planelles, Paola Pinto-Hernández, Juan Castilla-Silgado, Almudena Coto-Vilcapoma, Sergio Díez-Robles, Noelia Blanco-Agudín, Cristina Tomás-Zapico, Eduardo Iglesias-Gutiérrez, Benjamín Fernández-García, Agustin F. Fernandez, Mario F. Fraga, Physical exercise shapes the mouse brain epigenome, Molecular Metabolism, Volume 54, 2021, 101398, ISSN 2212-8778, https://doi.org/10.1016/j.molmet.2021.101398.
NO Los autores desean agradecer a Ronnie Lendrum por la edición del manuscrito. Este trabajo contó con el apoyo de la Asociación Española Contra el Cáncer (PROYE18061FERN a MFF), la cofinanciación 2018-2022/FEDER del Gobierno de Asturias (PCTI) (IDI/2018/146 a MFF), la Fundación General CSIC (0348_CIE_6_E a MFF), el Instituto de Salud Carlos III (Plan Nacional de I+D+I) que cofinancia FEDER (PI18/01527 a MFF y AFF), el MINECO (DEP2015-69980-P a BFG), y la Fundación Tatiana Pérez de Guzmán el Bueno (“Ayudas a Proyectos de Investigación en Neurociencia-2020” a CTZ y EIG). RGU cuenta con el apoyo del Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER) . JRT cuenta con el apoyo de una beca Juan de la Cierva del Ministerio de Ciencia e Innovación español MCIN/AEI /10.13039/501100011033 (IJC2018-036825-I). RFP cuenta con el apoyo del programa Severo Ochoa (BP17-114). PPH cuenta con el apoyo por Ayudas para la realización de Tesis Doctorales . Modalidad Beca de la Universidad de Oviedo (PAPI-20-PF-19). También agradecemos el apoyo del IUOPA-ISPA-FINBA (el IUOPA cuenta con el apoyo de la Obra Social Cajastur-Liberbank , España).
NO Asociación Española Contra el Cáncer
NO FEDER del Gobierno de Asturias
NO Instituto de Salud Carlos III (Plan Nacional de I+D+I)
DS Docta Complutense
RD 7 abr 2025