RT Journal Article
T1 Phytotherapeutic potential of Lamiaceae essential oils and their monoterpenes against Giardia duodenalis.
A1 Marcos-Herraiz, Sara
A1 Alonso Fernández, Sara
A1 Irisarri, María José
A1 Arroyo Díaz, Jaime
A1 Ponce Gordo, Francisco
A1 González-Coloma, Azucena
A1 Navarro Rocha, Juliana
A1 Azami Conesa, Iris
A1 Gómez Muñoz, María Teresa
A1 Bailén, María
AB Lamiaceae and Asteraceae plant species have been widely used in Mediterranean ethnomedicine for gastrointestinal disorders. They are also known for their antioxidant, anti-inflammatory, anti-bacterial, anti-parasite, and anti-virus properties. Giardia duodenalis is the most prevalent intestinal protozoon in children and young dogs worldwide. Its zoonotic potential and frequent therapeutic failures with nitroimidazoles underscore the urgent need for alternative treatments. This study investigated the antigiardial activity of essential oils (EOs) from 22 medicinal plants belonging to Lamiaceae and Asteraceae, together with their major constituents. EO composition was determined by a metabolomic approach (GC-MS). Parasite metabolic activity was assessed using the MTT assay, and ultrastructural changes were examined by Transmission Electron Microscopy. The strongest antigiardial effects were observed with Lavandula luisieri, Thymus vulgaris, Mentha suaveolens, Satureja montana (IC50 <25), L. lanata, and T. zygis, (IC50= 27.9-71.5 µg/ml). The highest selective indexes were obtained with γ-terpinene, caryophyllene oxide, carvacrol and thymol (SI≥1.3-2.4). Synergistic interactions were detected with linalyl acetate and linalool (present in Lavandula EOs), linalyl acetate with ρ-cymene or thymol, or combinations of ρ-cymene, γ-terpinene, thymol, and carvacrol (present in Satureja EOs). Transmission Electron Microscopy revealed membranolysis, enlarged periplasmic vacuoles, and cytoplasmic loss in trophozoites exposed to γ-terpinene after 1 h. These findings provide phytotherapeutic evidence supporting essential oils from Lavandula, Mentha, Thymus, and Satureja as promising antigiardial agents. Their main components γ-terpinene, caryophyllene oxide, carvacrol and thymol could have potential applications in veterinary parasitology
PB Elsevier
SN 0304-4017
YR 2026
FD 2026
LK https://hdl.handle.net/20.500.14352/131529
UL https://hdl.handle.net/20.500.14352/131529
LA eng
NO Marcos-Herraiz, S., Alonso Fernández, S., Irisarri, M. J., Arroyo Díaz, J., Ponce-Gordo, F., González-Coloma, A., Navarro Rocha, J., Azami-Conesa, I., Gómez-Muñoz, M. T., & Bailén, M. (2026). Phytotherapeutic potential of Lamiaceae essential oils and their monoterpenes against Giardia duodenalis. Veterinary parasitology, 343, 110702. Advance online publication. https://doi.org/10.1016/j.vetpar.2026.110702
NO Credit authorship contribution statement Alonso Fernandez ´ Sara: Writing – review & editing, Methodology, Formal analysis, Data curation. Irisarri María jose: ´ Writing – review & editing, Methodology, Formal analysis, Data curation. María Teresa Gomez-Mu ´ noz: ˜ Writing – review & editing, Writing – original draft, Validation, Supervision, Funding acquisition, Formal analysis, Data curation, Conceptualization. Sara Marcos-Herraiz: Writing – review & editing, Supervision, Software, Methodology, Formal analysis, Data curation. Azucena Gonzalez-Coloma: ´ Writing – review & editing, Supervision, Investigation, Funding acquisition, Conceptualization. Juliana Navarro Rocha: Writing – review & editing, Resources, Methodology, Formal analysis, Data curation. Jaime Arroyo Díaz: Writing – review & editing, Methodology, Formal analysis, Data curation. Francisco Ponce-Gordo: Writing – review & editing, Methodology, Investigation, Formal analysis, Data curation. Iris Azami-Conesa: Writing – review & editing, Methodology, Investigation, Formal analysis. María Bailen: ´ Writing – review & editing, Validation, Supervision, Methodology, Investigation, Formal analysis, Conceptualization.
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 23 abr 2026