RT Journal Article
T1 CdSe quantum dots for two-photon fluorescence thermal imaging
A1 Martínez Maestro, Laura
A1 Rodríguez, Emma
A1 Rodríguez, Francisco
A1 Iglesias de La Cruz, María del Carmen
A1 Juarranz, Angeles
A1 Naccache, Rafik
A1 Vetrone, Fiorenzo
A1 Jaque, Daniel
A1 Capobianco, John
A1 Solé, José
AB The technological development of quantum dots has ushered in a new era in fluorescence bioimaging, which was propelled with the advent of novel multiphoton fluorescence microscopes. Here, the potential use of CdSe quantum dots has been evaluated as fluorescent nanothermometers for two-photon fluorescence microscopy. In addition to the enhancement in spatial resolution inherent to any multiphoton excitation processes, two-photon (near-infrared) excitation leads to a temperature sensitivity of the emission intensity much higher than that achieved under one-photon (visible) excitation. The peak emission wavelength is also temperature sensitive, providing an additional approach for thermal imaging, which is particularly interesting for systems where nanoparticles are not homogeneously dispersed. On the basis of these superior thermal sensitivity properties of the two-photon excited fluorescence, we have demonstrated the ability of CdSe quantum dots to image a temperature gradient artificially created in a biocompatible fluid (phosphate-buffered saline) and also their ability to measure an intracellular temperature increase externally induced in a single living cell.
PB American Chemical Society
SN 1530-6984
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/97448
UL https://hdl.handle.net/20.500.14352/97448
LA eng
NO Maestro, Laura Martinez, et al. «CdSe Quantum Dots for Two-Photon Fluorescence Thermal Imaging». Nano Letters, vol. 10, n.o 12, diciembre de 2010, pp. 5109-15. https://doi.org/10.1021/nl1036098.
NO Universidad Autonoma de Madrid
NO Comunidad de Madrid
NO Ministerio de Educación y Ciencia (España)
NO Natural Sciences and Engineering Research Council of Canada
NO Ministere du Developpement Economique, de l'Innovation et de l'Exportation (Quebec)
DS Docta Complutense
RD 22 ene 2026