RT Journal Article
T1 Arsenic species-binding proteins in human cardiovascular and muscle tissues
A1 Pizarro, I.
A1 Roman Silva, D.A.
A1 Gómez Gómez, María Milagros
A1 Cámara Rica, Carmen
A1 Palacios Corvillo, M.Antonia
AB The intracellular As-protein binding in cytosol and methanol–water extract of the auricle and saphene tissues of As impacted people was evaluated by bidimensional size exclusion FPLC-UV-ICP-MS. The fractionation of cytosol using Superdex, Phenomenex and MonoQ HR 5/5 columns, shows that As was distributed in a wide range of contiguous fractions of each column, being 8, 25, 50 % the percentages of As in the collected fractions, respectively. Arsenic a sulphur coelute when FPLC–UV–ICP–MS was applied, which could implicate that As is bound to bio-compounds of different molecular mass through vicinal sulphur groups. The monitoring of S, Cu and P. In the methanol: water extracts a similar study than performed with the cytosol using preparative gel chromatography on Sephadex G-75 and Shephadex G-100 columns. A very low As and protein contain were found in the different fractions of both SEC fractionating series. A similar As–protein association to that found in the cytosol after fractionating with MonoQ HR 5/5 was observed for auricle and saphene. Inorganic and methylated As speciation in the 20 - 26 cytosol fractions obtained within the Phenomenex column was performed by HPLC–ICP–MS using the Hamilton PRP-X100 column. Only As(III) and As(V) were present and the results obtained shows that the As(III)/As(V) ratio is constant in most cases. Direct evidence of the existence of As–binding peptides in auricle and saphene vein from arsenic impacted human beings has have been obtained which was previously reported by means of novo peptide synthesis.
PB Chilean Chemical Society
SN 0717-9707
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/42728
UL https://hdl.handle.net/20.500.14352/42728
LA eng
NO EU´s RTD programme
DS Docta Complutense
RD 17 abr 2025