RT Journal Article
T1 Mobile integrons encode phage defense systems
A1 Kieffer, Nicolas
A1 Hipólito Carrillo de Albornoz, Alberto
A1 Ortiz Miravalles, Laura
A1 Blanco Torres, Paula
A1 Delobelle, Thomas
A1 Vizuete, Patricia
A1 Ojeda, Francisco Manuel
A1 Jové, Thomas
A1 Jurenas, Dukas
A1 García Quintanilla, Meritxell
A1 Carvalho, André
A1 Domingo Calap, Pilar
A1 Escudero García-Calderón, José Antonio
AB Integrons are bacterial genetic elements that capture, stockpile,and modulate the expression of genes encoded in integroncassettes. Mobile integrons (MIs) are borne on plasmids, actingas a vehicle for hundreds of antimicrobial resistance genesamong key pathogens. These elements also carry genecassettes of unknown function (gcus) whose role and adaptivevalue remain unexplored. In this work, we show that gcusencode phage resistance systems, many of which are newlydiscovered. Bacteriophage resistance integron cassettes(BRiCs) can be combined and mixed with resistance cassettesto produce multiphage or drug and phage resistance. Thefitness costs of BRiCs are variable and dependent on thegenetic context and can be modulated by changing the order ofcassettes in the array. Hence, MIs act as highly mobile, low-costdefense islands.
PB American Association for the Advancement of Science
SN 0036-8075
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/120482
UL https://hdl.handle.net/20.500.14352/120482
LA eng
NO Kieffer, N., Hipólito, A., Ortiz-Miravalles, L., Blanco, P., Delobelle, T., Vizuete, P., Ojeda, F. M., Jové, T., Jurenas, D., García-Quintanilla, M., Carvalho, A., Domingo-Calap, P., & Escudero, J. A. (2025). Mobile integrons encode phage defense systems. Science (New York, N.Y.), 388(6747), eads0915. https://doi.org/10.1126/science.ads0915
NO Author contributions: Conceptualization: J.A.E.; Investigation: N.K., A.H., L.O.M., P.B., T.D., P.V., F.M.O., T.J., M.G.-Q., A.C., P.D.-C.; Funding acquisition: J.A.E.; Project administration: J.A.E.; Supervision: M.G.-Q., P.D.-C., J.A.E.; Writing — original draft: N.K., A.H., J.A.E.; Writing — review & editing: N.K., A.H., D.J., P.D.-C., M.G.-Q., J.A.E
NO European Commission
NO Universidad Complutense
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 20 jul 2025