RT Journal Article
T1 Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis
A1 Sánchez-Jaut, Sandra
A1 Pérez-Benavente, Susana
A1 Abad, Paloma
A1 Méndez Cuadro, Darío
A1 Puyet Catalina, Antonio
A1 Díez Martín, Amalia
A1 Galicia Poblet, Gonzalo
A1 Gómez Domínguez, Elena
A1 Moran Jiménez, María J.
A1 Bautista Santa Cruz, José Manuel
A1 Azcárate, Isabel G.
AB Iron overload caused by hereditary hemochromatosis (HH) increases free reactive oxygen species that, in turn, induce lipid peroxidation. Its 4-hydroxynonenal (HNE) by-product is a wellestablished marker of lipid peroxidation since it reacts with accessible proteins with deleterious consequences. Indeed, elevated levels of HNE are often detected in a wide variety of human diseases related to oxidative stress. Here, we evaluated HNE-modified proteins in the membrane of erythrocytes from HH patients and in organs of Hfe−/− male and female mice, a mouse model of HH. For this purpose, we used one- and two-dimensional gel electrophoresis, immunoblotting and MALDI-TOF/TOF analysis. We identified cytoskeletal membrane proteins and membrane receptors of erythrocytes bound to HNE exclusively in HH patients. Furthermore, kidney and brain of Hfe−/−mice contained more HNE-adducted protein than healthy controls. Our results identified main HNE-modified proteins suggesting that HH favours preferred protein targets for oxidation by HNE.
SN 1422-0067
YR 2023
FD 2023-02-02
LK https://hdl.handle.net/20.500.14352/94131
UL https://hdl.handle.net/20.500.14352/94131
LA eng
NO Sánchez-Jaut, S.; Pérez-Benavente, S.; Abad, P.; Méndez-Cuadro, D.; Puyet, A.; Diez, A.; Galicia-Poblet, G.; Gómez-Domínguez, E.; Moran-Jiménez, M.J.; Bautista, J.M.; et al. Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis. Int. J. Mol. Sci. 2023, 24, 2922. https:// doi.org/10.3390/ijms24032922
NO Ramón Areces Foundation.
DS Docta Complutense
RD 7 abr 2025