RT Journal Article
T1 Inside human aortic stenosis: a proteomic analysis of plasma
A1 Gil Dones, Félix
A1 Darde, Verónica
A1 Alonso-Orgaz, Sergio
A1 Lopez-Almodovar, Luis
A1 Mourino-Alvarez, Laura
A1 Padial, Luis
A1 Vivanco, Fernando
A1 Barderas, Maria
AB Valvular aortic stenosis (AS) produces a slowly progressive obstruction in left ventricular outflow track. For this reason, aortic valve replacement is warranted when the valvular stenosis is hemodinamically significant, becoming the most common worldwide cause of aortic valve surgery. Recent epidemiologic studies have revealed an association between degenerative AS and cardiovascular risk factors for atherosclerosis, althought reducing the exposure to such factors and statin therapies both fail to delay or reverse the pathology. Hence, a deeper understanding of the pathophysiology of this disease is required to identify appropriate preventive measures. A proteomic analysis of plasma will permit to know and identify the changes in protein expression induced by AS in this tissue. Using two-dimensional difference gel electrophoresis (2D-DIGE) followed by mass spectrometry (MS), we compared the crude (not pre-fractioned) and pre-fractioned plasma from AS patients and control subjects. We sought to identify plasma proteins whose expression is modified in AS. In addition we investigated if crude plasma presented some alterations in the more abundant proteins since to date, has never been studied before. We also further investigated the link between this disease and atherosclerosis with a view to identifying new potential markers and therapeutic targets.
PB Elsevier
SN 1874-3919
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/94604
UL https://hdl.handle.net/20.500.14352/94604
LA eng
NO Gil-Dones, Félix, et al. «Inside Human Aortic Stenosis: A Proteomic Analysis of Plasma». Journal of Proteomics, vol. 75, n.o 5, febrero de 2012, pp. 1639-53. https://doi.org/10.1016/j.jprot.2011.11.036.
NO This work was supported by grants from the Instituto de Salud Carlos III (FIS PI070537, CP09/00229), Fondo de Investigación Sanitaria de Castilla la Mancha (FISCAM, PI2008/28 and PI2008/08) and Redes Temáticas de Investigación Cooperativa (FONDOS FEDER, RD06/0014/1015), CNIC.
NO Instituto de Salud Carlos III
NO Junta de Comunidades de Castilla-La Mancha
NO European Commission
DS Docta Complutense
RD 6 oct 2024