RT Journal Article
T1 Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe
A1 Monopoli, Marco
A1 Zendrini, Andrea
A1 Wu, Dan
A1 Cheung, Shane
A1 Durán Sampedro, Gonzalo
A1 French, Brendan
A1 Nolan, John
A1 Piskareva, Olga
A1 Stalings, Raymond
A1 Ducoli, Serena
A1 Bergese, Paolo
A1 O’Shea, Donal
AB The recognition of the biological, diagnostic andmedical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes.
PB Royal Society of Chemistry
SN 1359-7345
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/95510
UL https://hdl.handle.net/20.500.14352/95510
LA eng
NO Monopoli, Marco P., et al. «Endogenous Exosome Labelling with an Amphiphilic NIR-Fluorescent Probe». Chemical Communications, vol. 54, n.o 52, 2018, pp. 7219-22. https://doi.org/10.1039/C8CC02135J.
NO Science Foundation Ireland
DS Docta Complutense
RD 10 abr 2025