%0 Book Section
%T Time-lapse video microscopy and single cell tracking to study neural cell behavior in vitro.
publisher Springer US
%D 2020
%U 1064-3745
%@ https://hdl.handle.net/20.500.14352/128631
%X A comprehensive understanding of the mechanisms controlling the behavior of cell populations with regenerative potential is the first step to design effective therapeutic strategies for many diseases. However, a precise description of the biological events involved, such as proliferation, differentiation, cell fate decisions, migration, or viability, may be hampered by the classical use of experiments based on end-point analysis. By contrast, live imaging and single cell tracking provides researchers with an accurate readout of these features in cells throughout an experiment. Here, we describe a protocol to apply time-lapse video microscopy and post-processing of the data to study critical aspects of the biology and the lineage progression of multiple neural populations.
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