RT Book, Section
T1 Time-lapse video microscopy and single cell tracking to study neural cell behavior in vitro.
A1 Paniagua Herranz, Lucía
A1 Gómez Villafuertes, María Rosa
A1 de Agustín Durán, David
A1 Gascón Jiménez, Sergio
A1 Pérez Sen, Raquel
A1 García Delicado, Esmerilda
A1 Miras Portugal, María Teresa
A1 Ortega De La O, Felipe
A2 Kursad Turksen, 
AB A comprehensive understanding of the mechanisms controlling the behavior of cell populations with regenerative potential is the first step to design effective therapeutic strategies for many diseases. However, a precise description of the biological events involved, such as proliferation, differentiation, cell fate decisions, migration, or viability, may be hampered by the classical use of experiments based on end-point analysis. By contrast, live imaging and single cell tracking provides researchers with an accurate readout of these features in cells throughout an experiment. Here, we describe a protocol to apply time-lapse video microscopy and post-processing of the data to study critical aspects of the biology and the lineage progression of multiple neural populations.
PB Springer US
SN 1064-3745
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/128631
UL https://hdl.handle.net/20.500.14352/128631
LA eng
NO Paniagua-Herranz L, Gómez-Villafuertes R, Agustín-Durán D, Gascón S, Pérez-Sen R, Delicado EG, Miras-Portugal MT, Ortega F (2020). Time-Lapse Video Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro. In: Turksen, K. (eds) Imaging and Tracking Stem Cells. Methods in Molecular Biology, vol 2150. Humana, New York, NY. https://doi.org/10.1007/7651_2019_219
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO Fundación Ramón Areces
DS Docta Complutense
RD 20 ene 2026