RT Journal Article
T1 Tonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat-inactivated Mycobacterium bovis
A1 Beltrán-Beck, Beatriz
A1 Boadella, Mariana
A1 Casal, Carmen
A1 Bezos Garrido, Javier
A1 Mazariegos Martínez-Peñalver, María
A1 Martín, MariPaz
A1 Galindo, Ruth C
A1 Pérez de la Lastra, José M
A1 Villar, Margarita
A1 Garrido, Joseba M
A1 Sevilla, Iker A
A1 Asensio, Fernando
A1 Sicilia, Javier
A1 Lyashchenko, Konstantin P
A1 Juste, Ramón A
A1 de la Fuente, José
A1 Gortázar, Christian
A1 Domínguez Rodríguez, Lucas José
A1 Romero Martínez, Beatriz
AB Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (Sus scrofa), to evaluate the use of several enzyme-linked immunosorbent assays (ELISAs) and lateral flow tests for in vivo TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-β), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in regions that are endemic for M. bovis and for TB vaccine research.
PB American Society for Microbiology
SN 1556-679X
YR 2014
FD 2014-08
LK https://hdl.handle.net/20.500.14352/35275
UL https://hdl.handle.net/20.500.14352/35275
LA eng
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 26 mar 2026