RT Journal Article
T1 Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1
A1 Arrieta, Ignacio
A1 Barrio, Manuel Del
A1 Vidarte, Luis
A1 Pozo, Victoria del
A1 Pastor Vargas, Carlos
A1 González Cabrero, Jesús
A1 Cárdaba, Blanca
A1 Rojo, Marta
A1 Mı́nguez, Ascensión
A1 Cortegano Jimeno, María Isabel
AB Ingestion of the parasitic nematode Anisakis simplex in undercooked fish can cause severe allergic reactions in some individuals. Using pooled human sera from sensitized patients we have probed an expression library for A. simplex antigens. One positive clone was found to encode a full length 21 kDa protein with strong homology to nematode troponins. The recombinant protein was expressed as a GST-fusion protein and found by immunoblot analysis to react with sera from 20% of allergic patients. The presence of functional EF-hand Ca(2+) binding motifs was demonstrated by gel-shift analysis.
PB Elsevier
SN 0166-6851
YR 2000
FD 2000
LK https://hdl.handle.net/20.500.14352/96205
UL https://hdl.handle.net/20.500.14352/96205
LA eng
NO Arrieta I, del Barrio M, Vidarte L, del Pozo V, Pastor C, Gonzalez-Cabrero J, Cárdaba B, Rojo M, Mínguez A, Cortegano I, Gallardo S, Aceituno E, Palomino P, Vivanco F, Lahoz C. Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1. Mol Biochem Parasitol. 2000 Apr 15;107(2):263-8. doi: 10.1016/s0166-6851(00)00192-4. PMID: 10779602.
NO Sociedad Española de Alergia e Inmunologı́a Clı́nica
DS Docta Complutense
RD 10 abr 2025