RT Journal Article
T1 Impact of Iron Deficiency on the Growth and Bioelectrical Profile of Different Gut Bacteria
A1 Quarta, Elisa
A1 Bourqqia-Ramzi, Marwane
A1 Muñoz Rodríguez, David
A1 García Esteban, María Teresa
A1 Murciano Cespedosa, Antonio
A1 Mateos González, Álvaro
A1 Conejero Meca, Francisco José
A1 Lombardo-Hernandez, Juan
A1 Mansilla-Guardiola, Jesús
A1 Baroni, Simona
A1 Geninatti Crich, Simonetta
A1 Geuna, Stefano
A1 Munaron, Luca
A1 Chiabrando, Deborah
A1 Herrera Rincón, Celia
AB Scope: Iron deficiency (ID) is the most common nutritional deficiency worldwide, impacting gut bacteria's metabolism and cellular biochemistry, but its effects on the microbiota-gut-brain axis (MGB) are poorly understood. Early-life ID-related dysbiosis is linked to neurodevelopmental impairments like autism and attention deficit hyperactivity disorder. Studying ID's impact on bacterial signaling can guide interventions to target MGB in iron-deficient populations. This study examined the responses of Escherichia coli (E. coli) and Limosilactobacillus reuteri (L. reuteri) to in-vitro ID conditions using the iron chelator 2,2’-Bipyridyl (BP). Methods and Results: We assessed and modeled their growth and cultivability and explored their bioelectric profiles using the voltage-sensitive dye DiBAC4(3). Results showed differential responses: L. reuteri's growth and cultivability were unaffected by BP, while E. coli's growth rate and cultivability decreased under ID. Additionally, we created a deterministic mathematical model that demonstrated a decrease in the population's average reproduction rate in E. coli under ID. Only E. coli exhibited an altered bioelectric profile, marked by increased cell depolarization in ID conditions, which was largely rescued upon the addition of a saturating concentration of iron. Conclusion: These findings highlight specific bioelectrical responses in gut bacteria to ID. Understanding this variability is crucial for deciphering the microbiota's role in health and disease, particularly concerning nutritional iron imbalance and bacterial signaling in the MGB.
PB Wiley
SN 2045-8827
YR 2025
FD 2025-06-25
LK https://hdl.handle.net/20.500.14352/123120
UL https://hdl.handle.net/20.500.14352/123120
LA eng
NO Quarta, E., Bourqqia‐Ramzi, M., Muñoz‐Rodriguez, D., García‐Esteban, M. T., Murciano‐Cespedosa, A., Mateos González, Álvaro, Conejero‐Meca, F. J., Lombardo‐Hernandez, J., Mansilla‐Guardiola, J., Baroni, S., Geninatti Crich, S., Geuna, S., Munaron, L., Chiabrando, D., & Herrera‐Rincon, C. (2025). Impact of Iron Deficiency on the Growth and Bioelectrical Profile of Different Gut Bacteria. MicrobiologyOpen, 14(3), e70015. https://doi.org/10.1002/mbo3.70015
NO Funding: This research was funded by the Ramon y Cajal program through the Spanish Ministry of Science, State Agency for Research (RYC2020‐029499‐I) and by the Complutense University of Madrid (Research Project PR3/23‐30827) to C.H.‐R. We also gratefully acknowledge support from the University of Turin (Grant for Internationalization) to D.C., and the New York University Shanghai.
NO Ministerio de Ciencia y Agencia Estatal de Investigación (España)
NO Universidad Complutense de Madrid
NO University of Turin
NO New York University Shanghai
DS Docta Complutense
RD 26 dic 2025