RT Journal Article
T1 Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii
A1 Morales Menchén, Ana
A1 Navarro García, Federico
A1 Guirao Abad, José P.
A1 Román González, Elvira
A1 Prieto Prieto, Antonio Daniel
A1 Coman, Ioana V
A1 Pla Alonso, Jesús
A1 Alonso Monge, Rebeca María Del Mar
AB Certain yeasts secrete peptides known as killer toxins or mycocins with a deleterious effect on sensitive yeasts or filamentous fungi, a common phenomenon in environmental species. In a recent work, different  () strains isolated from a wide variety of cheeses were identified as producing killer toxins active against  and . We have analyzed the killer activity of these toxins in  mutants defective in MAPK signaling pathways and found that the lack of the MAPK Hog1 (but not Cek1 or Mkc1) renders cells hypersensitive to  mycocins while mutants lacking other upstream elements of the pathway behave as the wild type strain. Point mutations in the phosphorylation site (T174A-176F) or in the kinase domain (K52R) of  gene showed that both activities were relevant for the survival of  to  killer toxins. Moreover, Hog1 phosphorylation was also required to sense and adapt to osmotic and oxidative stress while the kinase activity was somehow dispensable. Although the addition of supernatant from the killer toxin- producing  242 strain (-242) induced a slight intracellular increase in Reactive Oxygen Species (ROS), overexpression of cytosolic catalase did not protect  against this mycocin. This supernatant induced an increase in intracellular glycerol concentration suggesting that this toxin triggers an osmotic stress. We also provide evidence of a correlation between sensitivity to -242 killer toxin and resistance to Congo red, suggesting cell wall specific alterations in sensitive strains.
SN 2235-2988
YR 2018
FD 2018-05-03
LK https://hdl.handle.net/20.500.14352/13366
UL https://hdl.handle.net/20.500.14352/13366
LA eng
NO Morales Menchén, A., Navarro García, F., Guirao Abad, J. P. et al. «Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii». Frontiers in Cellular and Infection Microbiology, vol. 8, mayo de 2018, p. 135. DOI.org (Crossref), https://doi.org/10.3389/fcimb.2018.00135.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Coordination of European Funding for Infectious Diseases Research
DS Docta Complutense
RD 13 oct 2024