RT Journal Article
T1 Liquid chromatographic analysis of the anticancer alkaloid luotonin A and some new derivatives in human serum samples
A1 González Ruiz, Víctor
A1 Mussardo, Pierluigi
A1 Corda, Elisa
A1 Girotti, Stefano
A1 Olives Barba, Ana Isabel
A1 Martín Carmona, María Antonia
AB The quantitation of the natural cytotoxic and anti‐inflammatory alkaloid luotonin A and five recently synthesized derivatives is described, constituting the first report of a HPLC method for the analysis of these compounds in human serum samples. The conditions for the chromatographic separation were optimized and the method was validated for the analysis of these compounds in biological samples according to international guidelines. An RP‐HPLC method with fluorimetric detection and a C 18 stationary phase was applied. Different ACN/water mobile phases were assayed, including 0–4% of a mobile phase modifier such as tetrahydrofuran, dioxane or tert‐butyl methyl ether. Isocratic and gradient elution conditions are compared. The influence of pH on the efficiency and resolution of the separation was also considered. The developed method was applied to the determination of luotonins in pooled human serum samples by gradient elution RP‐HPLC using a simple cleanup procedure. The proposed chromatographic method exhibits satisfactory analytical figures of merit, with LOD from 1.0×10−10 to 2.0×10−10 M, intraday and interday precision below 6% except for the concentration level closest to LOD, and good agreement between experimental and theoretical concentrations. Therefore, the developed method is suitable, reliable, rapid, and simple.
SN 1615-9306
SN 1615-9314
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/99774
UL https://hdl.handle.net/20.500.14352/99774
LA eng
NO González‐Ruiz, Víctor, et al. «Liquid Chromatographic Analysis of the Anticancer Alkaloid Luotonin A and Some New Derivatives in Human Serum Samples». Journal of Separation Science, vol. 33, n.o 14, julio de 2010, pp. 2086-93. DOI.org (Crossref), https://doi.org/10.1002/jssc.201000175.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 7 abr 2025