RT Journal Article
T1 Active DNA unwinding dynamics during processive DNA replication
A1 Morin, Jose A.
A1 Cao García, Francisco Javier
A1 Lazaro, Jose M.
A1 Ricardo Arias-Gonzalez, J.
A1 Valpuesta, Jose M.
A1 Carrascosa, Jose L.
A1 Salas, Margarita
A1 Ibarra, Borja
AB Duplication of double-stranded DNA (dsDNA) requires a fine-tuned coordination between the DNA replication and unwinding reactions. Using optical tweezers, we probed the coupling dynamics between these two activities when they are simultaneously carried out by individual Phi29 DNA polymerase molecules replicating a dsDNA hairpin. We used the wild-type and an unwinding deficient polymerase variant and found that mechanical tension applied on the DNA and the DNA sequence modulate in different ways the replication, unwinding rates, and pause kinetics of each polymerase. However, incorporation of pause kinetics in a model to quantify the unwinding reaction reveals that both polymerases destabilize the fork with the same active mechanism and offers insights into the topological strategies that could be used by the Phi29 DNA polymerase and other DNA replication systems to couple unwinding and replication reactions.
PB National Academy of Sciences
SN 0027-8424
YR 2012
FD 2012-05-22
LK https://hdl.handle.net/20.500.14352/105439
UL https://hdl.handle.net/20.500.14352/105439
LA eng
NO Morin, J. A., Cao, F. J., Lázaro, J. M., Arias-Gonzalez, J. R., Valpuesta, J. M., Carrascosa, J. L., ... & Ibarra, B. (2012). Active DNA unwinding dynamics during processive DNA replication. Proceedings of the National Academy of Sciences, 109(21), 8115-8120.
NO Se deposita la versión postprint del artículo
NO Ministerio de Educación y Ciencia (España)
NO European Commission
DS Docta Complutense
RD 9 abr 2025