RT Journal Article
T1 Vascular catheter colonization: surveillance based on culture of needleless connectors
A1 Pérez Granda, María Jesús
A1 Guembe, María
A1 Cruces, Raquel
A1 Bouza Santiago, Emilio
AB BackgroundSuperficial culture has a high negative predictive value in the assessment of catheter tip colonization (CC) and catheter-related bloodstream infection (C-RBSI). However, the process of hub culture requires the hubs to be swabbed, and this carries a risk of dislodging the biofilm. At present, most catheter hubs are closed by needleless connectors (NCs) that are periodically replaced. Our objective was to compare the yield of SC (skin + hub culture) with that of skin + NC culture in the assessment of CC and C-RBSI.MethodsDuring 5 months, we included the patients on the Major Heart Surgery ICU when a central venous catheter (CVC) remained in place ≥7 days after insertion. SCs were taken simultaneously when the NC was withdrawn and processed by the semi-quantitative method, even when the catheter was not removed. All catheter tips were cultured. All NCs belonging to a single catheter lumen were individually flushed with 100 μl of brain-heart infusion (BHI) broth. We considered the lumen to be colonized when ≥1 NC culture from the lumen flush was positive. We collected a total of 60 catheters.ResultsThe overall CC rate was 15.0 %, and we confirmed two episodes of C-RBSI. The validity values after the comparison of SCs with skin + NC culture for prediction of CC were the following: sensitivity 66.7 % vs. 77.8 %, and negative predictive value 93.6 % vs. 93.1 %. The sensitivity and negative predictive value for prediction of C-RBSI was 100 % for both SC and skin + NC culture.ConclusionThe combination of skin and flushed NC culture can be an alternative to conventional SC for ruling out CC and C-RBSI.
PB BioMed Central Ltd
SN 1364-8535
YR 2016
FD 2016-05-28
LK https://hdl.handle.net/20.500.14352/120428
UL https://hdl.handle.net/20.500.14352/120428
LA eng
NO Pérez-Granda MJ, Guembe M, Cruces R, Bouza E. Vascular catheter colonization: Surveillance based on culture of needleless connectors. Critical Care. 2016;20(1).
NO M. Guembe cuenta con el apoyo del Programa Miguel Servet (ISCIII-MICINN, CP13/00268) del Fondo de Investigación en Salud (FIS) del Instituto de Salud Carlos III (ISCIII), Madrid, España, financiado parcialmente por el Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa”. Los financiadores no tuvieron ningún papel en el diseño del estudio, la recopilación y análisis de datos, la decisión de publicación o la preparación del manuscrito.
DS Docta Complutense
RD 7 jun 2025