RT Book, Section
T1 Assessing autophagy flux in glioblastoma temozolomide resistant cells
A1 Clark, Courtney
A1 Barzegar Behrooz, Amir
A1 Cordani, Marco
A1 Shojaei, Shahla
A1 Ghavami, Saeid
A2 Turksen, Kursad
AB Autophagy is a critical cellular process involved in the degradation and recycling of cytoplasmic components, playing a dual role in cancer by either promoting cell survival or facilitating cell death. In glioblastoma (GB), autophagy has been implicated in resistance to the chemotherapeutic agent temozolomide (TMZ). This study presents a novel method to accurately measure autophagy flux in TMZ-resistant glioblastoma cells, combining advanced imaging techniques with biochemical assays. By quantifying key autophagy markers such as LC3-II and SQSTM1, our approach provides detailed insights into the dynamic processes of autophagosome formation and clearance under therapeutic stress. This method advances our understanding of autophagy in GB chemoresistance and has significant implications for the development of autophagy-targeted therapies. The ability to monitor and manipulate autophagy flux in real time offers a promising avenue for monitoring and understanding TMZ resistance and improving patient outcomes in glioblastoma treatment.
PB Springer Nature
SN 9781071642672
SN 9781071642689
SN 1064-3745
YR 2024
FD 2024-07
LK https://hdl.handle.net/20.500.14352/119827
UL https://hdl.handle.net/20.500.14352/119827
LA eng
NO Clark, C., Barzegar Behrooz, A., Cordani, M., Shojaei, S., Ghavami, S. (2024). Assessing Autophagy Flux in Glioblastoma Temozolomide Resistant Cells. In: Turksen, K. (eds) Autophagy in Development and Disease. Methods in Molecular Biology, vol 2879. Humana, New York, NY. https://doi.org/10.1007/7651_2024_571
NO Funding:M.C. is supported by grant RYC2021-031003I funded by MICIU/AEI/https://doi.org/10.13039/501100011033 and by European Union NextGenerationEU/PRTR.
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Agencia Estatal de Investigación
DS Docta Complutense
RD 17 may 2025