%0 Journal Article
%A Gosálvez Berenguer, Jaime
%A Crespo Castejón, Francisco
%A Gutiérrez Cepeda, Luna
%A Serres Dalmau, María Consolacion
%A Jonhnston, Stephen
%T Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation
%D 2013
%@ 0936-6768
%U https://hdl.handle.net/20.500.14352/97263
%X Stallion spermatozoa recovered and examined immediatelyafter colloidal centrifugation resulted in a higher straight-linevelocity (VSL) than sperm processed using direct conven-tional  centrifugation  (p = 0.000),  but  there  was  nodifferences  in  the  progressive  motility  or  sperm  DNAfragmentation (SDF) as determined by the sperm chromatindispersion assay. However, when centrifuged spermatozoawere incubated at 37°C for 24 h to determine the rate of SDF(r-SDF), a lower r-SDF (p = 0.0011) was observed in thosesperm recovered after colloidal separation (0.5 ± 0.1%⁄h)compared to direct (1.2 ± 0.4%⁄h) or no centrifugation (r-SDF = 1.2 ± 0.3%⁄h). These results confirm that colloidalseparation of stallion spermatozoa results in prolonged spermDNA longevity, but these differences were only apparentfollowing a period of incubation and dynamic assessment.Consequently,  we  strongly  recommend  the  use  of  thedynamic form of the SDF assay for evaluating centrifugationand⁄or otherex vivoprocedures, as a single basal assessmentof SDF may inadvertently result in a false-positive evaluationof DNA quality.
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